H2O2 Biosensor Based on Poly(thionine) and Layer-by-Layer Self-assembly of Carbon Nanotubes and HRP

被引：0

作者：

Yang Shaoming ^{[1
]}

Huang Aihua ^{[1
]}

Zha Wenling ^{[1
]}

Wei Zhipeng ^{[1
]}

Zheng Longzhen ^{[1
]}

机构：

[1] E China Jiaotong Univ, Sch Basic Sci, Dept Chem & Chem Engn, Nanchang 330013, Peoples R China

来源：

ACTA CHIMICA SINICA | 2011年 / 69卷 / 18期

关键词：

poly(thionine); electro-polymerization; layer-by-layer self-assembly; biosensor; H2O2; HYDROGEN-PEROXIDE; HORSERADISH-PEROXIDASE; AMPEROMETRIC BIOSENSOR; IMMOBILIZATION; NANOPARTICLES;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Carbon nanotubes (CNTs)/horseradish peroxidase (HRP) multilayer reagentless H2O2 biosensor based on poly(thionine) (PTH) mediator was fabricated by the combination of electro-polymerization and layer-by-layer self-assembly method. Electrochemical impedance spectroscopy was used to monitor the layer-by-layer self-assembly process of CNTs/HRP multilayer. The electrochemical behavior of the HRP biosensor was studied by the cyclic voltammetry (CV) and chronoamperometry (I-t). The effect of the number of assembled enzyme layers, work potential, pH value and CNTs were investigated on the response of HRP electrode. The biosensor shows a good linear range of 2.0 X 10(-6)similar to 6.29 X 10(-3) mol/L for H2O2, R=0.998, with a detection limit of 1.0 X 10(-6) mol/L.

引用

页码：2143 / 2147

页数：5

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