Malachite Green Derivatives for Two-Photon RNA Detection

被引：20
作者：
Lux, Jacques [1 ]
Pena, Eduardo Jose [2 ]
Bolze, Frederic [1 ]
Heinlein, Manfred [2 ]
Nicoud, Jean-Francois [1 ]
机构：
[1] UMR 7213 UdS CNRS, Lab Biophoton & Pharmacol, F-67401 Illkirch Graffenstaden, France
[2] CNRS UPR 2357, Inst Biol Mol Plantes, F-67084 Strasbourg, France
来源：
CHEMBIOCHEM | 2012年 / 13卷 / 08期
关键词：
aptamers; fluorescent probes; malachite green; RNA visualization; two-photon absorption; LEUCOMALACHITE GREEN; APTAMER; LOCALIZATION; RECOGNITION; BINDING; PHOTOCHEMISTRY; INACTIVATION; FLUORESCENCE; ABSORPTION; MOLECULES;
D O I：
10.1002/cbic.201100747
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The design, preparation and characterisation of a library of malachite green (MG) derivatives for two-photon RNA labelling is described. Some of these MG derivatives exhibit an increased affinity for an MG-aptamer, as well as improved two-photon sensitivity when compared to the classical malachite green chloride. The underlying mechanisms and potential benefits for in vivo RNA visualisation are discussed.
引用
收藏
页码：1206 / 1213
页数：8
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