Monitoring hemodynamics and oxygenation of the kidney in rats by a combined near-infrared spectroscopy and invasive probe approach

被引：0

作者：

Grosenick, Dirk ^{[1
]}

Cantow, Kathleen ^{[2
]}

Arakelyan, Karen ^{[2
,3
]}

Wabnitz, Heidrun ^{[1
]}

Flemming, Bert ^{[2
]}

Skalweit, Angela ^{[2
]}

Ladwig, Mechthild ^{[2
]}

Macdonald, Rainer ^{[1
]}

Niendorf, Thoralf ^{[3
]}

Seeliger, Erdmann ^{[2
]}

机构：

[1] PTB, D-10587 Berlin, Germany

[2] Charite, Inst Vegetat Physiol, D-10115 Berlin, Germany

[3] Max Delbrueck Ctr Mol Med, BUFF, D-13125 Berlin, Germany

来源：

DIFFUSE OPTICAL IMAGING V | 2015年 / 9538卷

关键词：

near-infrared spectroscopy; kidney; oxygenation; perfusion; x-ray contrast medium; small animal;

D O I：

10.1117/12.2183417

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

We have developed a hybrid approach to investigate the dynamics of perfusion and oxygenation in the kidney of rats under pathophysiologically relevant conditions. Our approach combines near-infrared spectroscopy to quantify hemoglobin concentration and oxygen saturation in the renal cortex, and an invasive probe method for measuring total renal blood flow by an ultrasonic probe, perfusion by laser-Doppler fluxmetry, and tissue oxygen tension via fluorescence quenching. Hemoglobin concentration and oxygen saturation were determined from experimental data by a Monte Carlo model. The hybrid approach was applied to investigate and compare temporal changes during several types of interventions such as arterial and venous occlusions, as well as hyperoxia, hypoxia and hypercapnia induced by different mixtures of the inspired gas. The approach was also applied to study the effects of the x-ray contrast medium iodixanol on the kidney.

引用

页数：5

共 8 条

[1] Real-time assessment of renal cortical microvascular perfusion heterogeneities using near-infrared laser speckle imaging [J].