Effects of von Willebrand factor concentration and platelet collision on shear-induced platelet activation

The binding of plasma von Willebrand factor (vWF) to platelet glycoprotein (GP) Ib alpha in a high shear stress field, and subsequent integrin-GPIIb/IIIa-vWF conjunction induces platelet aggregation (SIPA). However,the specific biomechanical mechanism of the vWF-GPIb interaction still remains to be elucidated.A parallel-plate rectangular flow chamber was built to simulate a stenopeic artery flow pattern. Using the flow chamber, we examined shear-induced platelet activation (SIPAct) at different vWF concentrations (5-25 mu g/ml) and several simulated stenotic high shear rates. P-selectin expression on the platelets and annexinV binding to the platelets were used as two markers of platelet activation.At different localized shear rates (3,000 s(-1)-9,500 S(-1)), the percentage of annexin V and P-selectin positive cells increased from 8.3 +/- 0.4% to 22.3 +/- 1.8% (p 0.05) and from 17.4 +/- 0.5% to 33.5 +/- 2.5% (p 0.05), respectively.As the vWF concentration increased from 5 mu g/ml to 25 mu g/ml, the annexin V binding rate increased from 7.2 +/- 0.6% to 53.4 +/- 3.8% (p 0.05), and P-selectin expression increased from 16.5 +/- 1.2% to 65.9 +/- 5.2% (p 0.05). A test in a uniform shear field using cone-plate viscometer rheometry showed that the platelet activation rate was proportional to the platelet concentration.This result suggests that platelet collision is one of the impact factors of SIPAct.