Tracking the dynamics of heterotrophs and nitrifiers in moving- bed biofilm reactors operated at different COD/N ratios

被引：41
作者：
Bassin, J. P. [1 ,2 ]
Abbas, B. [1 ]
Vilela, C. L. S. [3 ]
Kleerebezem, R. [1 ]
Muyzer, G. [1 ]
Rosado, A. S. [3 ]
van Loosdrecht, M. C. M. [1 ]
Dezotti, M. [2 ]
机构：
[1] Delft Univ Technol, Dept Biotechnol, Delft, Netherlands
[2] Univ Fed Rio de Janeiro, Chem Engn Program, BR-21941972 Rio De Janeiro, Brazil
[3] Univ Fed Rio de Janeiro, Inst Microbiol Prof Paulo Goes, BR-21941972 Rio De Janeiro, Brazil
来源：
BIORESOURCE TECHNOLOGY | 2015年 / 192卷
关键词：
Microbial diversity; MBBR; Nitrification; FISH; DGGE; IN-SITU HYBRIDIZATION; WASTE-WATER TREATMENT; MICROBIAL COMMUNITY; NITRIFICATION; AMMONIA; GROWTH; POPULATION; INHIBITION; DIVERSITY; MBBR;
D O I：
10.1016/j.biortech.2015.05.051
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
In this study, the impact of COD/N ratio and feeding regime on the dynamics of heterotrophs and nitrifiers in moving-bed biofilm reactors was addressed. Based on DGGE analysis of 16S rRNA genes, the influent COD was found to be the main factor determining the overall bacterial diversity. The amoA-gene-based analysis suggested that the dynamic behavior of the substrate in continuous and pulse-feeding reactors influenced the selection of specific ammonium-oxidizing bacteria (AOB) strains. Furthermore, AOB diversity was directly related to the applied COD/N ratio and ammonium-nitrogen load. Maximum specific ammonium oxidation rates observed under non-substrate-limiting conditions were observed to be proportional to the fraction of nitrifiers within the bacterial community. FISH analysis revealed that Nitrosomonas genus dominated the AOB community in all reactors. Moreover, Nitrospira was found to be the only nitrite-oxidizing bacteria (NOB) in the fully autotrophic system, whereas Nitrobacter represented the dominant NOB genus in the organic carbon-fed reactors. (C) 2015 Elsevier Ltd. All rights reserved.
引用
收藏
页码：131 / 141
页数：11
相关论文
共 34 条
  • [1] [Anonymous], 2005, Standard methods for the examination of water and waste- water
  • [2] ANTHONISEN AC, 1976, J WATER POLLUT CON F, V48, P835
  • [3] Effect of Different Operational Conditions on Biofilm Development, Nitrification, and Nitrifying Microbial Population in Moving-Bed Biofilm Reactors
    Bassin, J. P.
    Kleerebezem, R.
    Rosado, A. S.
    van Loosdrecht, M. C. M.
    Dezotti, M.
    [J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2012, 46 (03) : 1546 - 1555
  • [4] Effect of Elevated Salt Concentrations on the Aerobic Granular Sludge Process: Linking Microbial Activity with Microbial Community Structure
    Bassin, J. P.
    Pronk, M.
    Muyzer, G.
    Kleerebezem, R.
    Dezotti, M.
    van Loosdrecht, M. C. M.
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2011, 77 (22) : 7942 - 7953
  • [5] Nitrification of industrial and domestic saline wastewaters in moving bed biofilm reactor and sequencing batch reactor
    Bassin, Joao P.
    Dezotti, Marcia
    Sant'Anna, Geraldo L., Jr.
    [J]. JOURNAL OF HAZARDOUS MATERIALS, 2011, 185 (01) : 242 - 248
  • [6] Effect of different salt adaptation strategies on the microbial diversity, activity, and settling of nitrifying sludge in sequencing batch reactors
    Bassin, Joao Paulo
    Kleerebezem, Robbert
    Muyzer, Gerard
    Rosado, Alexandre Soares
    van Loosdrecht, Mark C. M.
    Dezotti, Marcia
    [J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2012, 93 (03) : 1281 - 1294
  • [7] GROWTH AND OXIDATION-KINETICS OF 3 GENERA OF AMMONIA OXIDIZING NITRIFIERS
    BELSER, LW
    SCHMIDT, EL
    [J]. FEMS MICROBIOLOGY LETTERS, 1980, 7 (03) : 213 - 216
  • [8] Microbial Community Composition and Dynamics of Moving Bed Biofilm Reactor Systems Treating Municipal Sewage
    Biswas, Kristi
    Turner, Susan J.
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2012, 78 (03) : 855 - 864
  • [9] Brock T.D., 1991, BIOL MICROORGANISMS, VSixth
  • [10] Burrell PC, 1998, APPL ENVIRON MICROB, V64, P1878
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