Tracking the dynamics of heterotrophs and nitrifiers in moving- bed biofilm reactors operated at different COD/N ratios

被引：42

作者：

Bassin, J. P. ^{[1
,2
]}

Abbas, B. ^{[1
]}

Vilela, C. L. S. ^{[3
]}

Kleerebezem, R. ^{[1
]}

Muyzer, G. ^{[1
]}

Rosado, A. S. ^{[3
]}

van Loosdrecht, M. C. M. ^{[1
]}

Dezotti, M. ^{[2
]}

机构：

[1] Delft Univ Technol, Dept Biotechnol, Delft, Netherlands

[2] Univ Fed Rio de Janeiro, Chem Engn Program, BR-21941972 Rio De Janeiro, Brazil

[3] Univ Fed Rio de Janeiro, Inst Microbiol Prof Paulo Goes, BR-21941972 Rio De Janeiro, Brazil

来源：

BIORESOURCE TECHNOLOGY | 2015年 / 192卷

关键词：

Microbial diversity; MBBR; Nitrification; FISH; DGGE; IN-SITU HYBRIDIZATION; WASTE-WATER TREATMENT; MICROBIAL COMMUNITY; NITRIFICATION; AMMONIA; GROWTH; POPULATION; INHIBITION; DIVERSITY; MBBR;

D O I：

10.1016/j.biortech.2015.05.051

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

In this study, the impact of COD/N ratio and feeding regime on the dynamics of heterotrophs and nitrifiers in moving-bed biofilm reactors was addressed. Based on DGGE analysis of 16S rRNA genes, the influent COD was found to be the main factor determining the overall bacterial diversity. The amoA-gene-based analysis suggested that the dynamic behavior of the substrate in continuous and pulse-feeding reactors influenced the selection of specific ammonium-oxidizing bacteria (AOB) strains. Furthermore, AOB diversity was directly related to the applied COD/N ratio and ammonium-nitrogen load. Maximum specific ammonium oxidation rates observed under non-substrate-limiting conditions were observed to be proportional to the fraction of nitrifiers within the bacterial community. FISH analysis revealed that Nitrosomonas genus dominated the AOB community in all reactors. Moreover, Nitrospira was found to be the only nitrite-oxidizing bacteria (NOB) in the fully autotrophic system, whereas Nitrobacter represented the dominant NOB genus in the organic carbon-fed reactors. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：131 / 141

页数：11

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