Establishment and characterization of a human ovarian sarcomatoid carcinoma cell line BUPH:OVSC

被引：2

作者：

Guo, H ^{[1
]}

Feng, J ^{[1
]}

Liu, G ^{[1
]}

Cui, H ^{[1
]}

Ye, X ^{[1
]}

Yao, Y ^{[1
]}

Fu, T ^{[1
]}

机构：

[1] Peking Univ, Peoples Hosp, Gynecol Oncol Ctr, Beijing 100044, Peoples R China

来源：

INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER | 2005年 / 15卷 / 05期

关键词：

cell line; ovarian tumor; primary culture; sarcomatoid carcinoma;

D O I：

10.1111/j.1525-1438.2005.00148.x

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We first established a human ovarian sarcomatoid carcinoma cell line designated BUPH:OVSC from primary culture. The specimen was derived from the mural nodule in an ovarian mucinous tumor and cultured in vitro. To date, the cell line has been maintained for over 100 passages. Its biologic characteristics were studied by light and electron microscopy, which revealed spindle-shaped or polygonal cells with a doubling time of 39.5 h. The agglutination test of BUPH: OVSC was positive, and cell colonies were formed in soft agar. Chromosome analysis revealed its karyotype to be a pseudodiploidy. One X chromosome deletion and chromosome 20 addition were detected, and aberrant chromosomes t (1q; 12q) and 14p(+) were its chromosome markers. BUPH: OVSC was tumorigenic in nude mice. Hematoxylin and eosin staining of transplanted tumors showed that the cells were morphologically sarcomatoid. However, the transmission electron microscopic observation exposed its epithelial origin. The cell line coexpresses cytokeratin and vimentin. It dose not appear to express estrogen and progesterone receptors or the CA125 tumor marker. Alcian blue/periodic acid-Schiff staining indicates that the cells could secrete acid mucopolysaccharide. In conclusion, BUPH: OVSC displays unique cellular properties, which make it a useful model for the study of human ovarian sarcomatoid carcinomas.

引用

页码：856 / 865

页数：10

共 41 条

[1] Establishment and characterization of three new cell lines derived from the ascites of human ovarian carcinomas [J].

Alama, A ;

Barbieri, F ;

Favre, A ;

Cagnoli, M ;

Noviello, E ;

Pedulla, F ;

Viale, M ;

Foglia, G ;

Ragni, N .

GYNECOLOGIC ONCOLOGY, 1996, 62 (01) :82-88

[2]

BRIERS TW, 1989, CANCER RES, V49, P5153

[3] IMMUNOHISTOLOGY OF A SARCOMATOUS MURAL NODULE IN AN OVARIAN MUCINOUS CYSTADENOCARCINOMA [J].

BRUIJN, JA ;

SMIT, VTHBM ;

QUE, DG ;

FLEUREN, GJ .

INTERNATIONAL JOURNAL OF GYNECOLOGICAL PATHOLOGY, 1987, 6 (03) :287-293

[4]

CZERNOBILSKY B, 1983, CANCER, V51, P141, DOI 10.1002/1097-0142(19830101)51:1<141::AID-CNCR2820510128>3.0.CO

[5]

2-7

[6]

DeNictolis M, 1990, SURG PATHOL, V3, P309

[7] SARCOMA-LIKE MURAL NODULES COMBINED WITH A MICROFOCUS OF ANAPLASTIC CARCINOMA IN MUCINOUS OVARIAN TUMOR [J].

FUJII, S ;

KONISHI, I ;

KOBAYASHI, F ;

OKAMURA, H ;

YAMABE, H ;

MORI, T .

GYNECOLOGIC ONCOLOGY, 1985, 20 (02) :219-233

[8] Cancer statistics, 2001 [J].

Greenlee, RT ;

Hill-Harmon, MB ;

Murray, T ;

Thun, M .

CA-A CANCER JOURNAL FOR CLINICIANS, 2001, 51 (01) :15-36

[9]

Guilbeau C, 2003, ANN PATHOL, V23, P340

[10] Ovarian mucinous cystadenoma associated with mural leiomyomatous nodule and massive ovarian edema [J].

Hameed, A ;

Ying, AJ ;

KeyhaniRofagha, S ;

Xie, DL ;

Copeland, LJ .

GYNECOLOGIC ONCOLOGY, 1997, 67 (02) :226-229

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