Subpopulation Composition and Activation of T Lymphocytes during Coculturing with Mesenchymal Stromal Cells in Medium with Different O2 Content

被引:6
作者
Buravkova, L. B. [1 ]
Grigorieva, O. G. [1 ]
Andreeva, E. R. [1 ]
Andrianova, I. V. [1 ]
Rylova, Yu. V. [1 ]
机构
[1] Russian Acad Sci, Inst Med & Biol Problems, Moscow, Russia
关键词
T lymphocytes; mesenchymal stromal precursor cells from adipose tissue; reduced oxygen content; STEM-CELLS; HYPOXIA; TISSUE;
D O I
10.1007/s10517-011-1326-8
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
The concentration of O-2 during coculturing practically did not affect the subpopulation composition of T lymphocytes (CD3(+)/CD4(+), CD3(+)/CD8(+), CD3(+)/CD16(+)/CD56(+) T cells) under conditions of PHA-induced activation. Coculturing with mesenchymal stromal cells (MSC) led to a significant decrease in the ratio of lymphocytes carrying activation markers (CD3(+)/CD25(+) and CD3(+)/HLA-DR+) and increase in the number of CD3(+)/CD16(+)/CD56(+) T cells. The percent of activated HLA-DR+ T cells in a heterotypic culture with MSC at 5% O-2 was much lower than that observed under normal conditions of culturing (20% O-2). Our results suggest that antigen presentation by T lymphocytes due to HLA-DR expression can be reduced in the target tissues at low concentration of O-2, while the interaction between allogeneic MSC probably contributes to more significant inhibition of the immune response.
引用
收藏
页码:344 / 346
页数:3
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