A Mitochondrial-Targeted Two-Photon Probe for Zinc Ion

被引：229

作者：

Masanta, Goutam ^{[1
]}

Lim, Chang Su ^{[3
]}

Kim, Hyung Joong ^{[2
]}

Han, Ji Hee ^{[3
]}

Kim, Hwan Myung ^{[1
,2
]}

Cho, Bong Rae ^{[3
]}

机构：

[1] Ajou Univ, Mol Sci & Technol Res Ctr, Suwon 443749, South Korea

[2] Ajou Univ, Div Energy Syst Res, Suwon 443749, South Korea

[3] Korea Univ, Dept Chem, Seoul 136701, South Korea

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2011年 / 133卷 / 15期

基金：

新加坡国家研究基金会;

关键词：

FLUORESCENT-PROBES; METAL-IONS; ZN2+; ALZHEIMERS; DISEASE; SENSORS; TISSUE; CELLS;

D O I：

10.1021/ja200444t

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report a two-photon probe (SZn-Mito) for mitochondrial zinc ions ([Zn2+](m)). This probe shows a 7-fold enhancement of two-photon-excited fluorescence in response to Zn2+ with a dissociation constant (K-d(TP)) of 3.1 +/- 0.1 nM and pH insensitivity in the biologically relevant range, allowing the detection of [Zn2+](m) in a rat hippocampal slice at a depth of 100-200 mu m without interference from other metal ions through the use of two-photon microscopy.

引用

页码：5698 / 5700

页数：3

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