Injectable, Interconnected, High-Porosity Macroporous Biocompatible Gelatin Scaffolds Made by Surfactant-Free Emulsion Templating

被引：57

作者：

Oh, Bernice H. L. ^{[1
,2
]}

Bismarck, Alexander ^{[2
,3
]}

Chan-Park, Mary B. ^{[1
]}

机构：

[1] Nanyang Technol Univ, Sch Chem & Biomed Engn, Singapore 637459, Singapore

[2] Univ London Imperial Coll Sci Technol & Med, Dept Chem Engn, Polymer & Composite Engn PaCE Grp, London SW7 2AZ, England

[3] Univ Vienna, Inst Mat Chem & Res, Polymer & Composite Engn PaCE Grp, Fac Chem, A-1090 Vienna, Austria

来源：

MACROMOLECULAR RAPID COMMUNICATIONS | 2015年 / 36卷 / 04期

基金：

英国工程与自然科学研究理事会;

关键词：

cell encapsulation; gelatin; high internal phase emulsions; hydrogels; self-emulsifying; thermoresponsive; CELL-CULTURE; IN-VITRO; TISSUE; BONE; POLYHIPES; STABILITY; POLYMERS; DESIGN; HYDROGELS; CARTILAGE;

D O I：

10.1002/marc.201400524

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

High-porosity interconnected, thermoresponsive macroporous hydrogels are prepared from oil-in-water high internal phase emulsions (HIPEs) stabilized by gelatin-graft-poly(N-isopropylacrylamide). PolyHIPEs are obtained by gelling HIPEs utilizing the thermoresponsiveness of the copolymer components. PolyHIPEs properties can be controlled by varying the aqueous phase composition, internal phase volume ratio, and gelation temperature. PolyHIPEs respond to temperature changes experienced during cell seeding, allowing fibroblasts to spread, proliferate, and penetrate into the scaffold. Encapsulated cells survive ejection of cell-laden hydrogels through a hypodermic needle. This system provides a new strategy for the fabrication of safe injectable biocompatible tissue engineering scaffolds.

引用

页码：364 / 372

页数：9

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