Expression of Bacterial GshF in Pichia pastoris for Glutathione Production

被引：20

作者：

Ge, Shaolin ^{[1
,2
]}

Zhu, Taicheng ^{[1
]}

Li, Yin ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Microbiol, Beijing, Peoples R China

[2] Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Peoples R China

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2012年 / 78卷 / 15期

基金：

中国国家自然科学基金;

关键词：

GAMMA-GLUTAMYLCYSTEINE SYNTHETASE; SACCHAROMYCES-CEREVISIAE; ESCHERICHIA-COLI; PROTEIN; GENE; BIOSYNTHESIS;

D O I：

10.1128/AEM.00509-12

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Conventionally, two consecutive enzymatic reactions catalyzed by gamma-glutamylcysteine synthetase and glutathione synthetase are most commonly used for glutathione production. Here we demonstrate that bacterial bifunctional GshF can be used for glutathione production in a eukaryotic system without accumulation of the intermediate gamma-glutamylcysteine.

引用

页码：5435 / 5439

页数：5

共 23 条

[1] Structural Basis for Feedback and Pharmacological Inhibition of Saccharomyces cerevisiae Glutamate Cysteine Ligase [J].

Biterova, Ekaterina I. ;

Barycki, Joseph J. .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2010, 285 (19) :14459-14466

[2] Mechanistic Details of Glutathione Biosynthesis Revealed by Crystal Structures of Saccharomyces cerevisiae Glutamate Cysteine Ligase [J].

Biterova, Ekaterina I. ;

Barycki, Joseph J. .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2009, 284 (47) :32700-32708

[3] EXPRESSION IN THE YEAST PICHIA PASTORIS [J].

Cregg, James M. ;

Tolstorukov, Ilya ;

Kusari, Anasua ;

Sunga, Jay ;

Madden, Knut ;

Chappell, Thomas .

GUIDE TO PROTEIN PURIFICATION, SECOND EDITION, 2009, 463 :169-189

[4] Expression of heterologous proteins in Pichia pastoris:: a useful experimental tool in protein engineering and production [J].

Daly, R ;

Hearn, MTW .

JOURNAL OF MOLECULAR RECOGNITION, 2005, 18 (02) :119-138

[5] Protein secretion in Pichia pastoris and advances in protein production [J].

Damasceno, Leonardo M. ;

Huang, Chung-Jr ;

Batt, Carl A. .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2012, 93 (01) :31-39

[6] Increasing glutathione formation by functional expression of the γ-glutamylcysteine synthetase gene in Saccharomyces cerevisiae [J].

Fan, XY ;

He, XP ;

Guo, XN ;

Qu, N ;

Wang, CL ;

Zhang, BR .

BIOTECHNOLOGY LETTERS, 2004, 26 (05) :415-417

[7] Improved glutathione production by gene expression in Pichia pastoris [J].

Fei, Liwen ;

Wang, Yan ;

Chen, Shaoxin .

BIOPROCESS AND BIOSYSTEMS ENGINEERING, 2009, 32 (06) :729-735

[8] A multidomain fusion protein in Listeria monocytogenes catalyzes the two primary activities for glutathione biosynthesis [J].

Gopal, S ;

Borovok, I ;

Ofer, A ;

Yanku, M ;

Cohen, G ;

Goebel, W ;

Kreft, E ;

Aharonowitz, Y .

JOURNAL OF BACTERIOLOGY, 2005, 187 (11) :3839-3847

[9]

Gushima H, 1983, J Appl Biochem, V5, P43

[10] Glutathione synthesis in Streptococcus agalactiae -: One protein accounts for γ-glutamylcysteine synthetase and glutathione synthetase activities [J].

Janowiak, BE ;

Griffith, OW .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (12) :11829-11839

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