Crystallization and preliminary X-ray diffraction analysis of shikimate kinase from Mycobacterium tuberculosis in complex with MgADP

被引：10

作者：

Gu, YJ

Reshetnikova, L

Li, Y

Yan, H

Singh, SV

Ji, XH ^{[1
]}

机构：

[1] NCI, Macromol Crystallog Lab, Frederick, MD 21702 USA

[2] Michigan State Univ, Dept Biochem & Mol Biol, E Lansing, MI 48824 USA

[3] Univ Pittsburgh, Sch Med, Dept Pharmacol, Pittsburgh, PA 15261 USA

[4] Univ Pittsburgh, Sch Med, Inst Canc, Pittsburgh, PA 15261 USA

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 2001年 / 57卷

关键词：

D O I：

10.1107/S0907444901014032

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Shikimate kinase (SK) from Mycobacterium tuberculosis (Mt) was overexpressed in Escherichia coli, purified and cocrystallized with MgADP in hanging drops using the vapor-diffusion procedure with PEG 4000 and 2-propanol as precipitants at pH 7.5. The crystal of MtSK-MgADP, which diffracted to 2.2 Angstrom resolution, belonged to space group P3(2)21 or P3(1)21, with unit-cell parameters a=b=64.01, c=92.41 Angstrom. There was one MtSK molecule in the asymmetric unit. Molecular-replacement trials with the crystal structure of SK from Erwinia chrysanthemi (PDB code 1shk) and adenylate kinase (PDB code 1ake) as search models were not successful. Heavy-atom derivative screening is in progress.

引用

页码：1870 / 1871

页数：2