HDV-like self-cleaving ribozymes

被引：76

作者：

Webb, Chiu-Ho T. ^{[1
]}

Luptak, Andrej ^{[1
,2
,3
]}

机构：

[1] Univ Calif Irvine, Dept Mol Biol & Biochem, Irvine, CA 92717 USA

[2] Univ Calif Irvine, Dept Chem, Irvine, CA 92717 USA

[3] Univ Calif Irvine, Dept Pharmaceut Sci, Irvine, CA USA

来源：

RNA BIOLOGY | 2011年 / 8卷 / 05期

关键词：

motif search; R2; retrotransposon; catalytic RNA; HEPATITIS-DELTA-VIRUS; IN-VITRO SELECTION; HAMMERHEAD RIBOZYME; CRYSTAL-STRUCTURE; RNA; CLEAVAGE; SEQUENCE; CPEB3; SITE; REPLICATION;

D O I：

10.4161/rna.8.5.16226

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

HDV ribozymes catalyze their own scission from the transcript during rolling circle replication of the hepatitis delta virus. In vitro selection of self-cleaving ribozymes from a human genomic library revealed an HDV-like ribozyme in the second intron of the human CPEB3 gene and recent results suggest that this RNA affects episodic memory performance. Bioinformatic searches based on the secondary structure of the HDV/CPEB3 fold yielded numerous functional ribozymes in a wide variety of organisms. Genomic mapping of these RNAs suggested several biological roles, one of which is the 5' processing of non-LTR retrotransposons. The family of HDV-like ribozymes thus continues to grow in numbers and biological importance.

引用

页码：719 / 727

页数：9

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