STAT3 and MITF cooperatively induce cellular transformation through upregulation of c-fos expression

被引:43
|
作者
Joo, A
Aburatani, H
Morii, E
Iba, H
Yoshimura, A
机构
[1] Kyushu Univ, Med Inst Bioregulat, Div Mol & Cellular Immunol, Higashi Ku, Fukuoka 8128582, Japan
[2] Univ Tokyo, Adv Sci & Technol Res Ctr, Div Genome Sci, Meguro Ku, Tokyo 1538904, Japan
[3] Osaka Univ, Sch Med, Dept Pathol, Osaka 5650871, Japan
[4] Univ Tokyo, Inst Med Sci, Minato Ku, Tokyo 1088639, Japan
基金
日本学术振兴会;
关键词
MITF; STAT3; c-fos; cellular transformation;
D O I
10.1038/sj.onc.1207174
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The signal transducer and activator of transcription ( STAT) family proteins are transcription factors critical in mediating cytokine signaling. Among them, STAT3 is frequently activated in a number of human cancers and transformed cell lines and is implicated in tumorigenesis. However, although constitutively activated STAT3 mutant (STAT3C) leads to cellular transformation, its transformation potential such as colony-forming activity in soft-agar is much weaker than that of v-src. To identify tumorigenic factors that cooperatively induce cellular transformation with STAT3C, we screened the retroviral cDNA library. We found that the microphthalmia-associated transcription factor ( MITF), an essential transcription factor for melanocyte development and pigmentation, induces anchorage-independent growth of NIH-3T3 cells in cooperation with STAT3C. Micro array analysis revealed that c-fos is highly expressed in transformants expressing STAT3C and MITF. Promoter analysis and chromatin immunoprecipitation assay suggested that both STAT3 and MITF can cooperatively upregulate the c-fos gene. In addition, the transformation of NIH-3T3 cells by both MITF and STAT3C was significantly suppressed by a dominant-negative AP-1 retrovirus. These data indicate that MITF and STAT3 cooperatively induce c-fos, resulting in cellular transformation.
引用
收藏
页码:726 / 734
页数:9
相关论文
共 50 条
  • [21] NF-κB and STAT3 cooperatively induce IL6 in starved cancer cells
    Yoon, S.
    Woo, S. U.
    Kang, J. H.
    Kim, K.
    Shin, H-J
    Gwak, H-S
    Park, S.
    Chwae, Y-J
    ONCOGENE, 2012, 31 (29) : 3467 - 3481
  • [22] Signaling pathways leading to transcription and translation cooperatively regulate the transient increase in expression of c-Fos protein
    Takeuchi, K
    Shibamoto, S
    Nagamine, K
    Shigemori, I
    Omura, S
    Kitamura, N
    Ito, F
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (28) : 26077 - 26083
  • [23] Stat3 activation is required for cellular transformation by v-src
    Bromberg, JF
    Horvath, CM
    Besser, D
    Lathem, WW
    Darnell, JE
    MOLECULAR AND CELLULAR BIOLOGY, 1998, 18 (05) : 2553 - 2558
  • [24] EXPRESSION OF C-FOS PROTEIN IN BRAIN - METABOLIC MAPPING AT THE CELLULAR-LEVEL
    SAGAR, SM
    SHARP, FR
    CURRAN, T
    SCIENCE, 1988, 240 (4857) : 1328 - 1331
  • [25] Cot, a novel kinase of histone H3, induces cellular transformation through up-regulation of c-fos transcriptional activity
    Choi, Hong Seok
    Kang, Bong Seok
    Shim, Jung-Hyun
    Cho, Yong-Yeon
    Choi, Bu Young
    Bode, Ann M.
    Dong, Zigang
    FASEB JOURNAL, 2008, 22 (01): : 113 - 126
  • [26] ROLE OF 3' UNTRANSLATED SEQUENCES IN THE CONTROL OF C-FOS EXPRESSION
    RUBINO, K
    CURRAN, T
    JOURNAL OF CELLULAR BIOCHEMISTRY, 1987, : 79 - 79
  • [27] Interleukin induces hepcidin expression through STAT3
    Wrighting, Diedra M.
    Andrews, Nancy C.
    BLOOD, 2006, 108 (09) : 3204 - 3209
  • [28] RECTAL AND COLONIC DISTENSION DIFFERENTIALLY INDUCE EXPRESSION OF C-FOS BUT NOT OF C-JUN IN DVC AND PVN
    MONNIKES, H
    FLUG, M
    TACHE, Y
    EISSELE, R
    STERNINI, C
    ARNOLD, R
    GASTROENTEROLOGY, 1993, 104 (04) : A841 - A841
  • [29] Clioquinol Increases the Expression of VGF, a Neuropeptide Precursor, Through Induction of c-Fos Expression
    Katsuyama, Masato
    Ibi, Masakazu
    Matsumoto, Misaki
    Iwata, Kazumi
    Ohshima, Yoichi
    Yabe-Nishimura, Chihiro
    JOURNAL OF PHARMACOLOGICAL SCIENCES, 2014, 124 (04) : 427 - 432
  • [30] Coordinate regulation of STAT signaling and c-fos expression by the tyrosine phosphatase SHP-2
    Servidei, T
    Aoki, Y
    Lewis, SE
    Symes, A
    Fink, JS
    Reeves, SA
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (11) : 6233 - 6241