Genetically tunable M13 phage films utilizing evaporating droplets

被引:9
作者
Alberts, Erik [1 ]
Warner, Chris [2 ]
Barnes, Eftihia [3 ]
Pilkiewicz, Kevin [2 ]
Perkins, Edward [2 ]
Poda, Aimee [2 ]
机构
[1] HX5 LLC, Vicksburg, MS 39180 USA
[2] US Army, Engineer Res & Dev Ctr, Environm Lab, Vicksburg, MS USA
[3] US Army, Engineer Res & Dev Ctr, Geotech & Struct Lab, Vicksburg, MS USA
关键词
Cellular matrix; Bacteriophage; Sessile droplets; Self-assembly; Biointerfaces; Coffee ring effect; COFFEE-RING; PATTERN-FORMATION; BACTERIOPHAGE; DYNAMICS; DEPOSITION; REVERSES; FLOW;
D O I
10.1016/j.colsurfb.2017.10.039
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
This effort utilizes a genetically tunable system of bacteriophage to evaluate the effect of charge, temperature and particle concentration on biomaterial synthesis utilizing the coffee ring (CR) effect. There was a 1.6-3 fold suppression of the CR at higher temperatures while maintaining self-assembled structures of thin films. This suppression was observed in phage with charged and uncharged surface chemistry, which formed ordered and disordered assemblies respectively, indicating CR suppression is not dependent on short-range ordering or surface chemistry. Analysis of the drying process suggests weakened capillary flow at elevated temperatures caused CR suppression and could be further enhanced for controlled assembly for advanced biomaterials. (C) 2017 The Authors. Published by Elsevier B.V.
引用
收藏
页码:210 / 218
页数:9
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