Isolation and identification of Arcobacter species from environmental and drinking water samples

被引：21

作者：

Talay, Funda ^{[1
]}

Molva, Celenk ^{[2
]}

Atabay, Halil Ibrahim ^{[2
,3
]}

机构：

[1] Izmir Inst Technol, Dept Bioengn & Biotechnol, TR-35430 Izmir, Turkey

[2] Izmir Inst Technol, Dept Food Engn, TR-35430 Izmir, Turkey

[3] Sifa Univ, Dept Med Microbiol, Fac Med, TR-35100 Izmir, Turkey

来源：

FOLIA MICROBIOLOGICA | 2016年 / 61卷 / 06期

关键词：

MULTIPLEX PCR; LAKE-ERIE; RAW-MILK; SPP; PREVALENCE; PATHOGEN; CAMPYLOBACTERS; BUTZLERI; CHICKEN; PROBES;

D O I：

10.1007/s12223-016-0460-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Water plays an important role in the transmission of Arcobacter spp. to animals and humans. The aim of this study was to isolate and characterize Arcobacter spp. from 115 different water samples (66 sewage, 25 rivers, 16 spring water, and 8 drinking water) in Izmir, Turkey. In total, 41 samples (35.7 %) were found positive for Arcobacter spp. by the genus-specific PCR. Arcobacter butzleri was detected in 39 out of 115 samples (33.9 %) including 24 sewage, 13 rivers, and 2 spring water. The remaining Arcobacter spp. (n = 2) isolates could not be identified by m-PCR and 16S rRNA gene sequencing. Based on the phenotypic characterization, most of the Arcobacter species (87.8 %) indicated weak catalase activity. In addition, there were differences in phenotypic patterns among isolated species during growth at 37 A degrees C under microaerobic and aerobic conditions, in the presence of 2 % (39/41) and 3.5 % (32/41) NaCl and 0.04 % TTC (39/41) and on MacConkey agar (38/41). The results of this study indicated that environmental water samples are common sources for Arcobacter spp. Therefore, effective control measures should be taken to protect human health.

引用

页码：479 / 484

页数：6

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