Structural reorganization renders enhanced metalloprotein stability

被引：1

作者：

Botelho, Hugo M. ^{[1
]}

Gomes, Claudio M. ^{[1
]}

机构：

[1] Univ Nova Lisboa, Inst Tecnol Quim & Biol, EAN, P-2785572 Oeiras, Portugal

来源：

CHEMICAL COMMUNICATIONS | 2011年 / 47卷 / 39期

关键词：

DESULFOVIBRIO-GIGAS RUBREDOXIN; CLOSTRIDIUM-PASTEURIANUM; MESOPHILIC RUBREDOXIN; CIRCULAR-DICHROISM; NMR STRUCTURE; PROTEINS; RESOLUTION; DYNAMICS; MODEL;

D O I：

10.1039/c1cc13354c

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The enhanced stability of a mesophilic metalloprotein was assessed using biophysical spectroscopies. Significant local structural inter-conversions during thermal insult account for a reorganization of the protein scaffold, without disturbing the active metal site. This cushioning mechanism is proposed to be a generic property of metalloproteins contributing to enhanced stability.

引用

页码：11149 / 11151

页数：3

共 20 条

[1] What vibrations tell us about proteins [J].

Barth, A ;

Zscherp, C .

QUARTERLY REVIEWS OF BIOPHYSICS, 2002, 35 (04) :369-430

[2] Thermal stability of Clostridium pasteurianum rubredoxin:: Deconvoluting the contributions of the metal site and the protein [J].

Bonomi, F ;

Fessas, D ;

Iametti, S ;

Kurtz, DM ;

Mazzini, S .

PROTEIN SCIENCE, 2000, 9 (12) :2413-2426

[3] Hydrogen bonds in rubredoxins from mesophilic and hyperthermophilic organisms [J].

Bougault, CM ;

Eidsness, MK ;

Prestegard, JH .

BIOCHEMISTRY, 2003, 42 (15) :4357-4372

[4] Unfolding mechanism of rubredoxin from Pyrococcus furiosus [J].

Cavagnero, S ;

Zhou, ZH ;

Adams, MWW ;

Chan, SI .

BIOCHEMISTRY, 1998, 37 (10) :3377-3385

[5] Crystal structure of rubredoxin from Desulfovibrio gigas to ultra-high 0.68Å resolution [J].

Chen, Chun-Jung ;

Lin, Yi-Hung ;

Huang, Yen-Chieh ;

Liu, Ming-Yih .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2006, 349 (01) :79-90

[6] SYNTHESIS AND CHARACTERIZATION OF DESULFOVIBRIO-GIGAS RUBREDOXIN AND RUBREDOXIN FRAGMENTS [J].

CHRISTENSEN, HEM ;

HAMMERSTADPEDERSEN, JM ;

HOLM, A ;

IVERSEN, G ;

JENSEN, MH ;

ULSTRUP, J .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1994, 224 (01) :97-101

[7] On the relation between native geometry and conformational plasticity [J].

Faisca, Patricia F. N. ;

Gomes, Claudio M. .

BIOPHYSICAL CHEMISTRY, 2008, 138 (03) :99-106

[8] Studies on the redox centers of the terminal oxidase from Desulfovibrio gigas and evidence for its interaction with rubredoxin [J].

Gomes, CM ;

Silva, G ;

Oliveira, S ;

LeGall, J ;

Liu, MY ;

Xavier, AV ;

RodriguesPousada, C ;

Teixeira, M .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (36) :22502-22508

[9] Molecular dynamics study of a hyperthermophilic and a mesophilic rubredoxin [J].

Grottesi, A ;

Ceruso, MA ;

Colosimo, A ;

Di Nola, A .

PROTEINS-STRUCTURE FUNCTION AND GENETICS, 2002, 46 (03) :287-294

[10] High-temperature solution NMR structure of TmCsp [J].

Jung, A ;

Bamann, C ;

Kremer, W ;

Kalbitzer, HR ;

Brunner, E .

PROTEIN SCIENCE, 2004, 13 (02) :342-350

← 1 2 →