Expression and purification of chicken beta interferon and its antivirus immunological activity

被引：20

作者：

Cai, Meihong ^{[1
,2
]}

Zhu, Feng ^{[1
]}

Shen, Pingping ^{[1
]}

机构：

[1] Nanjing Univ, State Key Lab Pharmaceut Biotechnol, Nanjing 210093, Jiangsu, Peoples R China

[2] Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Peoples R China

来源：

PROTEIN EXPRESSION AND PURIFICATION | 2012年 / 84卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Interferon beta; Expression; VSV; IBDV; Pathological lesion; VIRUS;

D O I：

10.1016/j.pep.2012.04.014

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Interferon beta (IFN-beta) belongs to a class of natural proteins that can inhibit virus replication. In this paper, nucleic acid sequence encoding chicken interferon beta (chIFN-beta) mature protein was cloned and highly expressed in Escherichia coli (E. coli) by 1 mM IPTG induction. The expressed chIFN-beta was about 20% of total bacterial protein. The recombinant protein in form of inclusion body was then solubilised, refolded and purified to a purity of greater than 95% by immobilized metal ion affinity chromatography (IMAC). This recombinant chIFN-beta could inhibit vesicular stomatitis Indiana virus (VSV) and infectious bursal disease virus (IBDV) replication in vitro. Animal experiments showed that the recombinant chIFN-beta could decrease pathological lesions caused by the IBDV in bursa of Fabricius. These results will provide useful information for further investigations on veterinary clinical applications and fundamental research. (c) 2012 Elsevier Inc. All rights reserved.

引用

页码：123 / 129

页数：7

共 14 条

[1]

[Anonymous], 1992, INTERFERON PRINCIPLE

[2] Interferon-β suppresses herpes simplex virus type 1 replication in trigeminal ganglion cells through an RNase L-dependent pathway [J].

Carr, DJJ ;

Al-Khatib, K ;

James, CM ;

Silverman, R .

JOURNAL OF NEUROIMMUNOLOGY, 2003, 141 (1-2) :40-46

[3] Oral Fingolimod or Intramuscular Interferon for Relapsing Multiple Sclerosis [J].

Cohen, Jeffrey A. ;

Barkhof, Frederik ;

Comi, Giancarlo ;

Hartung, Hans-Peter ;

Khatri, Bhupendra O. ;

Montalban, Xavier ;

Pelletier, Jean ;

Capra, Ruggero ;

Gallo, Paolo ;

Izquierdo, Guillermo ;

Tiel-Wilck, Klaus ;

de Vera, Ana ;

Jin, James ;

Stites, Tracy ;

Wu, Stacy ;

Aradhye, Shreeram ;

Kappos, Ludwig .

NEW ENGLAND JOURNAL OF MEDICINE, 2010, 362 (05) :402-415

[4]

Han C.L., 2006, CHIN J VET MED, V42, P3

[5]

Inokuchi M., 2011, J VIRAL HEPATITUS, P1365

[6]

KAKUMU S, 1993, HEPATOLOGY, V18, P258, DOI 10.1016/0270-9139(93)90005-8

[7]

Kuga C., 2011, HEPATOLOGY, V873, P870

[8] Inhibition of PKR by vaccinia virus: role of the N- and C-terminal domains of E3L [J].

Langland, JO ;

Jacobs, BL .

VIROLOGY, 2004, 324 (02) :419-429

[9] High expression of glycogen-debranching enzyme in Escherichia coli and its competent purification method [J].

Nakayama, A ;

Yamamoto, K ;

Tabata, S .

PROTEIN EXPRESSION AND PURIFICATION, 2000, 19 (02) :298-303

[10] Cloning, expression, isotope labeling, purification, and characterization of bovine antimicrobial peptide, lactophoricin in Escherichia coli [J].

Park, Tae-Joon ;

Kim, Ji-Sun ;

Choi, Sung-Sub ;

Kim, Yongae .

PROTEIN EXPRESSION AND PURIFICATION, 2009, 65 (01) :23-29

← 1 2 →