A centrifugal microfluidic device for screening protein crystallization conditions by vapor diffusion

被引:18
作者
Wang, Li [1 ]
Sun, Kailei [2 ]
Hu, Xiaojian [2 ]
Li, Gang [1 ,3 ]
Jin, Qinghui [1 ]
Zhao, Jianlong [1 ]
机构
[1] Chinese Acad Sci, Shanghai Inst Microsyst & Informat Technol, State Key Lab Transducer Technol, Shanghai 200050, Peoples R China
[2] Fudan Univ, Sch Life Sci, Shanghai 200433, Peoples R China
[3] Chongqing Univ, Sch Optoelect Engn, Chongqing 400044, Peoples R China
来源
SENSORS AND ACTUATORS B-CHEMICAL | 2015年 / 219卷
基金
中国国家自然科学基金;
关键词
Vapor diffusion; Microfluidic chip; Protein crystallization; Centrifugal force; CAPILLARY STOP VALVES; SYSTEM; OPTIMIZATION; FABRICATION; MICROBATCH; CHALLENGE;
D O I
10.1016/j.snb.2015.04.105
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
This paper describes a centrifuge-based microfluidic device for performing nanoliter-scale protein crystallization trials by vapor diffusion. By combining the capillary effect and centrifugal force, this device spontaneously and parallelly meters and dispenses protein samples and precipitants into 24 reaction chambers for crystallization screening. Furthermore, the design of two-level capillary stop valves and vapor-diffusion chambers in this device allows vapor transport between the protein droplet and the stored precipitant, perfectly equivalent to that observed in conventional vapor diffusion techniques, which slowly brings the protein droplet into a supersaturation state to nucleate and grow protein crystals. We demonstrate the power of this device by testing it for rapid screening of protein crystallization conditions. Compared with the conventional hanging-drop technique, this device can identify more crystallization conditions and provide a wider range of supersaturation rates, while using two orders of magnitude less protein sample. This centrifuge-based microfluidic device provides a simple and low-cost tool for individual laboratories to rapidly screen initial protein crystallization conditions. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码:105 / 111
页数:7
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