The microcephaly gene aspm is involved in brain development in zebrafish

被引：21

作者：

Kim, Hyun-Taek ^{[2
]}

Lee, Mi-Sun ^{[2
]}

Choi, Jung-Hwa ^{[2
]}

Jung, Ju-Yeon

Ahn, Dae-Gwon ^{[2
]}

Yeo, Sang-Yeob ^{[3
]}

Choi, Dong-Kug ^{[1
]}

Kim, Cheol-Hee ^{[2
]}

机构：

[1] Konkuk Univ, Dept Biotechnol, Chungju 380701, South Korea

[2] Chungnam Natl Univ, Dept Biol & GRAST, Taejon 305764, South Korea

[3] Hanbat Natl Univ, Dept Biotechnol, Div Appl Chem & Biotechnol, Taejon 305719, South Korea

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2011年 / 409卷 / 04期

基金：

新加坡国家研究基金会;

关键词：

Microcephaly; ASPM; CNS; Mitotic arrest; Zebrafish; CEREBRAL CORTICAL SIZE; ABNORMAL SPINDLE; CENTROSOMAL PROTEIN; MAJOR DETERMINANT; MITOTIC SPINDLE; DROSOPHILA; MUTATIONS; IDENTIFICATION; ENCODES;

D O I：

10.1016/j.bbrc.2011.05.056

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

MCPH is a neurodevelopmental disorder characterized by a global reduction in cerebral cortical volume. Homozygous mutation of the MCPH5 gene, also known as ASPM, is the most common cause of the MCPH phenotype. To elucidate the roles of ASPM during embryonic development, the zebrafish aspm was identified, which is specifically expressed in proliferating cells in the CNS. Morpholino-mediated knock-down of aspm resulted in a significant reduction in head size. Furthermore, aspm-deficient embryos exhibited a mitotic arrest during early development. These findings suggest that the reduction in brain size in MCPH might be caused by lack of aspm function in the mitotic cell cycle and demonstrate that the zebrafish can provide a model system for congenital diseases of the human nervous system. (C) 2011 Elsevier Inc. All rights reserved.

引用

页码：640 / 644

页数：5

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