Influence of Placental Mannose/N-Acetyl Glucosamine-Binding Proteins on the Interaction of Insulin and Insulin-Like Growth Factors with Their Receptors

被引:0
作者
Nedic, O. [1 ]
Filimonovic, D. [2 ]
Mikovic, Z. [2 ]
Masnikosa, R. [1 ]
机构
[1] Univ Belgrade, Inst Applicat Nucl Energy, Belgrade 11080, Serbia
[2] Univ Belgrade, Clin Gynecol & Obstet Narodni Front, Belgrade 11000, Serbia
关键词
insulin; insulin-like growth factors; receptors; lectins; placenta; LECTIN; OLIGOSACCHARIDES; FORMS;
D O I
10.1134/S0006297911090033
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Placenta is a source of carbohydrate-binding proteins that function as molecular scavengers, but they could also be involved in interactions that assist in metabolic control. Mannose/N-acetyl-glucosamine (Man/GlcNAc)-binding proteins from placenta were isolated and their reactivity towards placental insulin and insulin-like growth factor receptors (IR and IGF-Rs) was analyzed. The lectins reduced the binding of insulin and IGF-I in a dose-dependent manner, while almost no effect was observed on the binding of IGF-II. The shape of the inhibition curves changed, suggesting altered binding specificity. The presence of sugar could not reverse completely the effect of the lectins, implicating both lectin-sugar and protein-protein conformational recognition. Since biological molecules in our experimental system were those that are in close relation in vivo, placental Man/GlcNAc-specific lectins may be regarded as potential allosteric modulators of ligand-receptor interactions in a system of homologous ligands, selectively affecting only binding to tyrosine kinase type receptors (IR and IGF-1R).
引用
收藏
页码:1003 / 1008
页数:6
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