Effect of naked and PEG-coated gold nanoparticles on histopathology and cytokines expression in rat liver and kidneys

被引：19
作者：
Al-Harbi, Najla S. [1 ]
Alrashood, Sara T. [2 ]
Siddiqi, Nikhat J. [1 ]
Arafah, Maha M. [3 ]
Ekhzaimy, Aishah [4 ]
Khan, Haseeb A. [1 ]
机构：
[1] King Saud Univ, Coll Sci, Dept Biochem, Riyadh 11451, Saudi Arabia
[2] King Saud Univ, Coll Pharm, Dept Pharmaceut Chem, Riyadh 11451, Saudi Arabia
[3] King Saud Univ, King Khalid Univ Hosp, Coll Med, Dept Pathol, Riyadh 12372, Saudi Arabia
[4] King Khalid Univ Hosp, Dept Med, Div Endocrinol, Riyadh 12372, Saudi Arabia
来源：
NANOMEDICINE | 2020年 / 15卷 / 03期
关键词：
gene expression; gold nanoparticles; histopathology; PEG-coating; proinflammatory cytokines; IN-VIVO TOXICITY; GENE-EXPRESSION; PROINFLAMMATORY CYTOKINES; HYALURONIC-ACID; LUNG-CANCER; BIODISTRIBUTION; SIZE; CYTOTOXICITY; GLUTATHIONE; ACTIVATION;
D O I：
10.2217/nnm-2019-0220
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Aim: To compare the effects of 5- and 50-nm naked and PEG-coated gold nanoparticles (AuNP) on proinflammatory cytokines (IL-1 beta, IL-6, TNF-alpha) expression and histopathological changes in liver and kidneys of rats. Materials & methods: Rats were injected with different nanoparticles and sacrificed after 24 h. Results: Both 5- and 50-nm AuNPs, and 50-nm PEG-AuNPs caused granular clumping of cytoplasm, edema and hydropic dystrophy in hepatic cells. Naked AuNPs of both sizes caused mild shrinkage, whereas 50-nm PEG-AuNPs enlarged the Bowman's space and capsule. Larger nanoparticles produced more profound mRNA expression of cytokines in both the organs. Conclusion: These findings suggest the roles of particle size and coating on immunological response and histopathological changes.
引用
收藏
页码：289 / 302
页数：14
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