One-step purification of epigallocatechin gallate from crude green tea extracts by isocratic hydrogen bond adsorption chromatography on β-cyclodextrin substituted agarose gel media

被引：17
作者：
Xu, J
Zhang, GF
Tan, TW
Janson, JC
机构：
[1] Uppsala Univ, Dept Surface Biotechnol, Uppsala Biomed Ctr, SE-75123 Uppsala, Sweden
[2] Chinese Acad Sci, Natl Key Lab Biochem Engn, Inst Proc Engn, Beijing 100080, Peoples R China
[3] Beijing Univ Chem Technol, Coll Life Sci & Technol, Dept Bioengn, Beijing 100029, Peoples R China
来源：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2005年 / 824卷 / 1-2期
基金：
中国国家自然科学基金; 高等学校博士学科点专项科研基金;
关键词：
beta-cyclodextrin; tea polyphenols; EGCG; catechin;
D O I：
10.1016/j.jchromb.2005.07.030
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
An oligomerized beta-cyclodextrin ligand coupled to brominated allyl-group substituted Sepharose HP has been used for the one-step purification of polyphenolic epigallocatechin gallate (EGCG), an important antioxidant, by isocratic hydrogen bond adsorption chromatography. With a sample load of 1.33 mg crude green tea polyphenolic extract per ml column packing and with water/ethanol/acetonitrile (57/30/13, v/v) as the optimum mobile phase, an EGCG purity of about 98% with a recovery of approximate 73% could be achieved by proper peak cutting. After about 10 sample applications, the column performance started to deteriorate but could be regenerated to its original function by cleaning with 0.35 M NaOH. (c) 2005 Elsevier B.V. All rights reserved.
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页码：323 / 326
页数：4
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