Colorectal cancer cell-derived CCL20 recruits regulatory T cells to promote chemoresistance via FOXO1/CEBPB/NF-κB signaling

被引:152
作者
Wang, Dan [1 ,2 ,3 ]
Yang, Li [1 ,2 ,3 ]
Yu, Weina [1 ,2 ,3 ]
Wu, Qian [1 ,2 ,3 ]
Lian, Jingyao [1 ,2 ,3 ]
Li, Feng [1 ,2 ,3 ]
Liu, Shasha [1 ,2 ,3 ]
Li, Aitian [1 ,2 ,3 ]
He, Zhiang [1 ,2 ,3 ]
Liu, Jinbo [4 ]
Sun, Zhenqiang [4 ]
Yuan, Weitang [4 ]
Zhang, Yi [1 ,2 ,3 ,5 ]
机构
[1] Zhengzhou Univ, Biotherapy Ctr, Affiliated Hosp 1, Zhengzhou 450052, Henan, Peoples R China
[2] Zhengzhou Univ, Canc Ctr, Affiliated Hosp 1, Zhengzhou 450052, Henan, Peoples R China
[3] Henan Key Lab Tumor Immunol & Biotherapy, Zhengzhou 450052, Henan, Peoples R China
[4] Zhengzhou Univ, Dept Anorectal Surg, Affiliated Hosp 1, Zhengzhou 450052, Henan, Peoples R China
[5] Zhengzhou Univ, Sch Life Sci, Zhengzhou 450001, Henan, Peoples R China
基金
中国国家自然科学基金;
关键词
Chemoresistance; CCL20; FOXO1/CEBPB/NF-kappa B; Regulatory T cells; Colorectal cancer (CRC);
D O I
10.1186/s40425-019-0701-2
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Background: Colorectal cancer (CRC) is one of the most common forms of cancer worldwide. The tumor microenvironment plays a key role in promoting the occurrence of chemoresistance in solid cancers. Effective targets to overcome resistance are necessary to improve the survival and prognosis of CRC patients. This study aimed to evaluate the molecular mechanisms of the tumor microenvironment that might be involved in chemoresistance in patients with CRC. Methods: We evaluated the effects of CCL20 on chemoresistance of CRC by recruitment of regulatory T cells (Tregs) in vitro and in vivo. Results: We found that the level of CCL20 derived from tumor cells was significantly higher in Folfox-resistant patients than in Folfox-sensitive patients. The high level of CCL20 was closely associated with chemoresistance and poor survival in CRC patients. Among the drugs in Folfox chemotherapy, we confirmed that 5-FU increased the expression of CCL20 in CRC. Moreover, CCL20 derived from 5-FU-resistant CRC cells promoted recruitment of Tregs. Tregs further enhanced the chemoresistance of CRC cells to 5-FU. FOXO1/CEBPB/NF-kappa B signaling was activated in CRC cells after 5-FU treatment and was required for CCL20 upregulation mediated by 5-FU. Furthermore, CCL20 blockade suppressed tumor progression and restored 5-FU sensitivity in CRC. Lastly, the expression of these signaling molecules mediating chemoresistance was closely correlated with poor survival of CRC patients. Conclusions: CRC cell-secreted CCL20 can recruit Tregs to promote chemoresistance via FOXO1/CEBPB/NF-kappa B signaling, indicating that the FOXO1/CEBPB/NF-kappa B/CCL20 axis might provide a promising target for CRC treatment.
引用
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页数:15
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