Effect of buffer system on the refolding of synthetic omega-conotoxin GVIA

被引：5

作者：

Flinn, JP ^{[1
]}

Murphy, R ^{[1
]}

机构：

[1] UNIV MELBOURNE,DEPT PHARMACOL,PARKVILLE,VIC 3052,AUSTRALIA

来源：

LETTERS IN PEPTIDE SCIENCE | 1996年 / 3卷 / 03期

关键词：

conotoxin; oxidative refolding; disulphide bridging; native fold; dimethyl sulphoxide;

D O I：

10.1007/BF00132972

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have shown that the yield of native omega-conotoxin GVIA after dimethylsulphoxide-mediated oxidative refolding of a synthetic, linear precursor is highly dependant upon the choice of refolding buffer and peptide concentration.

引用

页码：113 / 116

页数：4

共 5 条

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[2]

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[4] SYNTHESIS AND CHARACTERIZATION OF A DISULFIDE BOND ISOMER OF OMEGA-CONOTOXIN GVIA [J].

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