Wnt proteins promote neuronal differentiation in neural stem cell culture

被引：125

作者：

Muroyama, Y

Kondoh, H

Takada, S

机构：

[1] Japan Sci & Technol Corp, ERATO, Kondoh Differentiat Signaling Project, Kinki Innovat Ctr,Sakyo Ku, Kyoto 6068305, Japan

[2] Osaka Univ, Grad Sch Frontier Biosci, Osaka 5650871, Japan

[3] Natl Inst Basic Biol, Okazaki Natl Res Inst, Ctr Integrat Biosci, Okazaki 4448585, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2004年 / 313卷 / 04期

基金：

日本学术振兴会;

关键词：

Wnt; neural stem cell; neurosphere; neuron; astrocyte; oligodendrocyte; neuronal differentiation;

D O I：

10.1016/j.bbrc.2003.12.023

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Wnt signaling is implicated in the control of cell growth and differentiation during CNS development from studies of mouse and chick models, but its action at the cellular level has been poorly understand. In this study, we examine the in vitro function of Wnt signaling in embryonic neural stem cells, dissociated from neurospheres derived from E11.5 mouse telencephalon. Conditioned media containing active Wnt-3a proteins are added to the neural stem cells and its effect on regeneration of neurospheres and differentiation into neuronal and glial cells was examined. Wnt-3a proteins inhibit regeneration of neurospheres, but promote differentiation into MAP2-positive neuronal cells. Wnt-3a proteins also increase the number of GFAP-positive astrocytes but suppress the number of oligodendroglial lineage cells expressing PDGFR or O4. These results indicate that Wnt-3a signaling can inhibit the maintenance of neural stem cells, but rather promote the differentiation of neural stem cells into several cell lineages. (C) 2003 Elsevier Inc. All rights reserved.

引用

页码：915 / 921

页数：7

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