Development of a micro-plate magnetic chemiluminescence enzyme immunoassay (MMCLEIA) for rapid- and high-throughput analysis of 17β-estradiol in water samples

被引：50
作者：
Zhao, LX [1 ]
Lin, JM [1 ]
机构：
[1] Chinese Acad Sci, State Key Lab Environm Chem & Ecotoxicol, Ecoenvironm Sci Res Ctr, Beijing 100085, Peoples R China
来源：
JOURNAL OF BIOTECHNOLOGY | 2005年 / 118卷 / 02期
基金：
中国国家自然科学基金;
关键词：
micro-plate magnetic separator; micro-plate magnetic chemiluminescence enzyme immunoassay (MMCLEIA); 17 beta-estradiol (E2); environmental water sample;
D O I：
10.1016/j.jbiotec.2005.02.023
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A novel method of micro-plate magnetic chemiluminescence enzyme immunoassay (MMCLEIA) for the screening of 17 beta-estradiol in water samples was proposed. It used the micro-plate magnetic separator designed by ourselves which can achieve the high-throughput analysis without the samples pre-treatment and the sensitive chemilummescence system of AMPPD-ALP system. The method showed specific recognition of estrogen, without cross-reactions for three other major estrogenic compounds (17 beta-estradiol (E2), estriol (E3), ethinyl (E2)) commonly found in water. The MMCLEIA was also especially suitable for the large-scale samples processing. The working range for 17 beta-estradiol was 10-3000 pg/ml. The assay sensitivity was 5.4 pg/ml. Both intra- and inter-assay had relative standard deviation of less 15%. The effect of several physico-chemical parameters, such as the ratio of antibody versus antigen, incubation time and the concentration of detergent were studied. This method has been successfully applied to the preliminary detection of the sea-water. Compared with the chemiluminescence enzyme-linked immumosorbed assay, the correlation was good. (C) 2005 Elsevier B.V. All rights reserved.
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收藏
页码：177 / 186
页数：10
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