Multifarious cholesterol lowering potential of lactic acid bacteria equipped with desired probiotic functional attributes

被引:35
作者
Bhat, Bilqeesa [1 ]
Bajaj, Bijender Kumar [1 ]
机构
[1] Univ Jammu, Sch Biotechnol, Jammu 180006, India
关键词
Probiotics; Hypocholesterolemia; Lactobacillus casei; Lactobacillus paracasei; Bile salt hydrolase; LACTOBACILLUS-PLANTARUM STRAINS; IN-VITRO EVALUATION; MECHANISMS; GROWTH;
D O I
10.1007/s13205-020-02183-8
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Lactic acid bacteria (LAB) isolates possessed functional probiotic attributes, such as high hydrophobicity and autoaggregation ability, coaggregation capability with bacterial pathogens, antimicrobial activity, antioxidant potential, and hypocholesterolemic effects. Selected potential probiotic LAB, i.e. Lactobacillus paracasei M3, L. casei M5, L. paracasei M7, and few others were studied for their ability to lower cholesterol using a number of methods viz. cholesterol assimilation, bile salt deconjugation, cholesterol co-precipitation, cholesterol adhesion to probiotic cell wall, and miceller sequestration of cholesterol. L. casei M5 showed maximum bile salt hydrolase (BSH) activity, and released 57.63 nmol of glycine/min, and was closely followed by LAB isolate M9 which generated 52.12 nmol of glycine/min. Sodium glycocholate was deconjugated by L. casei M5 to produce 27.77 mu mol/mL of cholic acid, while other isolates produced 20-26 mu mol/mL of cholic acid. Cholesterol was assimilated significantly by isolate M6 (82.15%) and L. casei M5 (76.51%). L. casei M5 showed higher cholesterol co-precipitation ability (50.16 mu g/mL) as compared to other LAB isolates (33-44 mu g/mL). Miceller cholesterol concentration was reduced maximally by LAB isolate M8 (87.5%), followed by isolates M5 (84.75%), M9 (84%), M10 (80%), and M37 (79%). Higher cell wall adhesion of cholesterol was realized by L. casei M5 (42.48 mu g/mL) than other LAB isolates (30-40 mu g/mL). Selected LAB probiotics demonstrated short chain fatty acid (acetate, propionate, and butyrate) producing ability, yet another way of probiotics-mediated cholesterol lowering.
引用
收藏
页数:16
相关论文
共 51 条
  • [1] Determining the probiotic potential of cholesterol-reducing Lactobacillus and Weissella strains isolated from gherkins (fermented cucumber) and south Indian fermented koozh
    Anandharaj, Marimuthu
    Sivasankari, Balayogan
    Santhanakaruppu, Rajendran
    Manimaran, Muthusamy
    Rani, Rizwana Parveen
    Sivakumar, Subramaniyan
    [J]. RESEARCH IN MICROBIOLOGY, 2015, 166 (05) : 428 - 439
  • [2] Phytase-Producing Potential and Other Functional Attributes of Lactic Acid Bacteria Isolates for Prospective Probiotic Applications
    Andrabi, Syed Tabia
    Bhat, Bilqeesa
    Gupta, Mahak
    Bajaj, Bijender Kumar
    [J]. PROBIOTICS AND ANTIMICROBIAL PROTEINS, 2016, 8 (03) : 121 - 129
  • [3] Bioprospecting for Functionally-Proficient Potential Probiotics
    Bajaj, Bijender Kumar
    Andrabi, Tabia
    Claes, Ingmar J. J.
    Lebeer, Sarah
    [J]. CURRENT NUTRITION & FOOD SCIENCE, 2014, 10 (04) : 251 - 263
  • [4] FUNCTIONAL MECHANISMS OF PROBIOTICS
    Bajaj, Bijender Kumar
    Claes, Ingmar J. J.
    Lebeer, Sarah
    [J]. JOURNAL OF MICROBIOLOGY BIOTECHNOLOGY AND FOOD SCIENCES, 2015, 4 (04): : 321 - 327
  • [5] Bhat B, 2019, INDIAN J EXP BIOL, V57, P73
  • [6] Hypocholesterolemic and bioactive potential of exopolysaccharide from a probiotic Enterococcus faecium K1 isolated from kalarei
    Bhat, Bilqeesa
    Bajaj, Bijender Kumar
    [J]. BIORESOURCE TECHNOLOGY, 2018, 254 : 264 - 267
  • [7] Bhat B, 2017, INDIAN J BIOCHEM BIO, V54, P191
  • [8] Effect of Pullulan on Physicochemical, Microbiological, and Sensor Quality of Yogurts
    Chlebowska-Smigiel, Anna
    Kycia, Katarzyna
    Neffe-Skocinska, Katarzyna
    Kieliszek, Marek
    Gniewosz, Malgorzata
    Kolozyn-Krajewska, Danuta
    [J]. CURRENT PHARMACEUTICAL BIOTECHNOLOGY, 2019, 20 (06) : 489 - 496
  • [9] The Effect of Pullulan on the Growth and Acidifying Activity of Selected Stool Microflora of Human
    Chlebowska-Smigiel, Anna
    Gniewosz, Malgorzata
    Kieliszek, Marek
    Bzducha-Wrobel, Anna
    [J]. CURRENT PHARMACEUTICAL BIOTECHNOLOGY, 2017, 18 (02) : 121 - 126
  • [10] Cholesterol-lowering effects of a putative probiotic strain Lactobacillus plantarum EM isolated from kimchi
    Choi, Eun Ah
    Chang, Hae Choon
    [J]. LWT-FOOD SCIENCE AND TECHNOLOGY, 2015, 62 (01) : 210 - 217