Detection of Oligomerization of Epstein-Barr virus Oncoprotein LMP1 by FRET-Based Probes

被引：0
|
作者：
Wu, Zhiwei [1 ,2 ]
Ye, Qing [3 ]
Li, Jing [3 ]
Huang, Yimei [1 ,2 ]
Lin, Yating [1 ,2 ]
Zheng, Mengmeng [1 ,2 ]
Xie, Shusen [1 ,2 ]
Lin, Juqiang [1 ,2 ]
机构：
[1] Fujian Normal Univ, MOE Key Lab Optoelect Sci & Technol Med, Fuzhou 350007, Fujian, Peoples R China
[2] Fujian Normal Univ, Fujian Prov Key Lab Photon Technol, Fuzhou 350007, Fujian, Peoples R China
[3] Fujian Med Univ, Fujian Prov Hosp, Prov Clin Coll, Dept Otolaryngol, Fuzhou 350001, Fujian, Peoples R China
来源：
OPTICS IN HEALTH CARE AND BIOMEDICAL OPTICS VIII | 2018年 / 10820卷
基金：
中国国家自然科学基金;
关键词：
nasopharyngeal carcinoma; LMP1; oligomerization; FRET; fluorescence protein; fusion protein; fluorescence spectra; fluorescence image; NASOPHARYNGEAL; METASTASIS;
D O I：
10.1117/12.2500650
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
In this study, two FRET-based probes are constructed to research oligomerization of Epstein-Barr virus Oncoprotein LMP1 in live cells. The images of wide-field fluorescence microscopy display that the majority of two LMP1-associated probes co-localized in internal perinuclear membranes. Furthermore, the fluorescence spectra of single cell co-expressed two probes indicated that the ratio of two emission peaks is around one, and the fluorescence spectra changed insignificantly during an hour observation. These findings indicated that LMP1/LMP1 interacted stably in live cells.
引用
收藏
页数：6
相关论文
共 50 条
  • [41] C-Terminal Farnesylation of UCH-L1 Plays a Role in Transport of Epstein-Barr Virus Primary Oncoprotein LMP1 to Exosomes
    Kobayashi, E.
    Aga, M.
    Kondo, S.
    Whitehurst, C.
    Yoshizaki, T.
    Pagano, J. S.
    Shackelford, J.
    MSPHERE, 2018, 3 (01):
  • [42] Lower Levels of Survivin in Patients with Leukemic Low Grade B-Cell Lymphomas Expressing LMP1 Oncoprotein of Epstein-Barr Virus
    Diamantopoulos, Panagiotis Theodorou
    Polonyphi, Katerina
    Sofotasioiu, Maria
    Galanopoulos, Athanassios
    Spanakis, Nikolaos
    Rassidakis, George
    Kouzis, Panagiotis
    Papadopoulou, Vassiliki
    Kalala, Fani
    Iliakis, Theodoros
    Vassilakopoulos, Theodoros
    Angelopoulou, Maria K.
    Siakantaris, Marina
    Terpos, Evangelos
    Variami, Eleni
    Kollia, Panagoula
    Pangalis, Gerassimos A.
    Viniou, Nora-Athina
    BLOOD, 2012, 120 (21)
  • [43] Analysis of Immunogenicity of Intracellular CTAR Fragments of Epstein-Barr Virus Latent Phase Protein LMP1
    Lomakin, Ya. A.
    Shmidt, A. A.
    Bobik, T. V.
    Chernov, A. S.
    Pyrkov, A. Yu.
    Aleksandrova, N. M.
    Okunola, D. O.
    Vaskina, M. I.
    Ponomarenko, N. A.
    Telegin, G. B.
    Dubina, M. V.
    Belogurov, A. A., Jr.
    BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE, 2017, 163 (06) : 766 - 771
  • [44] Modified Anoikis Assay That Functionally Segregates Epstein-Barr Virus LMP1 Strains into Two Groups
    Wasil, Laura R.
    Shair, Kathy H. Y.
    JOURNAL OF VIROLOGY, 2018, 92 (18)
  • [45] Immune Surveillance and Therapy of Lymphomas Driven by Epstein-Barr Virus Protein LMP1 in a Mouse Model
    Zhang, Baochun
    Kracker, Sven
    Yasuda, Tomoharu
    Casola, Stefano
    Vanneman, Matthew
    Homig-Holzel, Cornelia
    Wang, Zhe
    Derudder, Emmanuel
    Li, Shuang
    Chakraborty, Tirtha
    Cotter, Shane E.
    Koyama, Shohei
    Currie, Treeve
    Freeman, Gordon J.
    Kutok, Jeffery L.
    Rodig, Scott J.
    Dranoff, Glenn
    Rajewsky, Klaus
    CELL, 2012, 148 (04) : 739 - 751
  • [46] Epstein-Barr virus miRNA-BART16 modulates cell proliferation by targeting LMP1
    Zhang, Yan
    Zhang, Wen
    Liu, Wen
    Liu, Hong
    Zhang, Yong
    Luo, Bing
    VIRUS RESEARCH, 2018, 256 : 38 - 44
  • [47] Molecular and functional analysis of the Epstein-Barr virus LMP1 oncogene promoter in lymphoproliferative diseases.
    Rothenberger, S
    Bachmann, E
    McQuain, C
    Odermatt, BF
    Knecht, H
    BLOOD, 1997, 90 (10) : 3611 - 3611
  • [48] The p38 Signaling Pathway Upregulates Expression of the Epstein-Barr Virus LMP1 Oncogene
    Johansson, Pegah
    Jansson, Ann
    Ruetschi, Ulla
    Rymo, Lars
    JOURNAL OF VIROLOGY, 2010, 84 (06) : 2787 - 2797
  • [49] Mimicry of CD40 signals by Epstein-Barr virus LMP1 in B lymphocyte responses
    Uchida, J
    Yasui, T
    Takaoka-Shichijo, Y
    Muraoka, M
    Kulwichit, W
    Raab-Traub, N
    Kikutani, H
    SCIENCE, 1999, 286 (5438) : 300 - 303
  • [50] Epstein-Barr virus LMP1 modulates the malignant potential of gastric carcinoma cells involving apoptosis
    Sheu, LF
    Chen, A
    Wei, YH
    Ho, KC
    Cheng, JY
    Meng, CL
    Lee, WH
    AMERICAN JOURNAL OF PATHOLOGY, 1998, 152 (01): : 63 - 74
12345