Detection of Oligomerization of Epstein-Barr virus Oncoprotein LMP1 by FRET-Based Probes

被引：0
|
作者：
Wu, Zhiwei [1 ,2 ]
Ye, Qing [3 ]
Li, Jing [3 ]
Huang, Yimei [1 ,2 ]
Lin, Yating [1 ,2 ]
Zheng, Mengmeng [1 ,2 ]
Xie, Shusen [1 ,2 ]
Lin, Juqiang [1 ,2 ]
机构：
[1] Fujian Normal Univ, MOE Key Lab Optoelect Sci & Technol Med, Fuzhou 350007, Fujian, Peoples R China
[2] Fujian Normal Univ, Fujian Prov Key Lab Photon Technol, Fuzhou 350007, Fujian, Peoples R China
[3] Fujian Med Univ, Fujian Prov Hosp, Prov Clin Coll, Dept Otolaryngol, Fuzhou 350001, Fujian, Peoples R China
来源：
OPTICS IN HEALTH CARE AND BIOMEDICAL OPTICS VIII | 2018年 / 10820卷
基金：
中国国家自然科学基金;
关键词：
nasopharyngeal carcinoma; LMP1; oligomerization; FRET; fluorescence protein; fusion protein; fluorescence spectra; fluorescence image; NASOPHARYNGEAL; METASTASIS;
D O I：
10.1117/12.2500650
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
In this study, two FRET-based probes are constructed to research oligomerization of Epstein-Barr virus Oncoprotein LMP1 in live cells. The images of wide-field fluorescence microscopy display that the majority of two LMP1-associated probes co-localized in internal perinuclear membranes. Furthermore, the fluorescence spectra of single cell co-expressed two probes indicated that the ratio of two emission peaks is around one, and the fluorescence spectra changed insignificantly during an hour observation. These findings indicated that LMP1/LMP1 interacted stably in live cells.
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页数：6
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