Detection of Oligomerization of Epstein-Barr virus Oncoprotein LMP1 by FRET-Based Probes

被引：0
|
作者：
Wu, Zhiwei [1 ,2 ]
Ye, Qing [3 ]
Li, Jing [3 ]
Huang, Yimei [1 ,2 ]
Lin, Yating [1 ,2 ]
Zheng, Mengmeng [1 ,2 ]
Xie, Shusen [1 ,2 ]
Lin, Juqiang [1 ,2 ]
机构：
[1] Fujian Normal Univ, MOE Key Lab Optoelect Sci & Technol Med, Fuzhou 350007, Fujian, Peoples R China
[2] Fujian Normal Univ, Fujian Prov Key Lab Photon Technol, Fuzhou 350007, Fujian, Peoples R China
[3] Fujian Med Univ, Fujian Prov Hosp, Prov Clin Coll, Dept Otolaryngol, Fuzhou 350001, Fujian, Peoples R China
来源：
OPTICS IN HEALTH CARE AND BIOMEDICAL OPTICS VIII | 2018年 / 10820卷
基金：
中国国家自然科学基金;
关键词：
nasopharyngeal carcinoma; LMP1; oligomerization; FRET; fluorescence protein; fusion protein; fluorescence spectra; fluorescence image; NASOPHARYNGEAL; METASTASIS;
D O I：
10.1117/12.2500650
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
In this study, two FRET-based probes are constructed to research oligomerization of Epstein-Barr virus Oncoprotein LMP1 in live cells. The images of wide-field fluorescence microscopy display that the majority of two LMP1-associated probes co-localized in internal perinuclear membranes. Furthermore, the fluorescence spectra of single cell co-expressed two probes indicated that the ratio of two emission peaks is around one, and the fluorescence spectra changed insignificantly during an hour observation. These findings indicated that LMP1/LMP1 interacted stably in live cells.
引用
收藏
页数：6
相关论文
共 50 条
  • [21] Induction of Epstein-Barr Virus Oncoprotein LMP1 by Transcription Factors AP-2 and Early B Cell Factor
    Murata, Takayuki
    Noda, Chieko
    Narita, Yohei
    Watanabe, Takahiro
    Yoshida, Masahiro
    Ashio, Keiji
    Sato, Yoshitaka
    Goshima, Fumi
    Kanda, Teru
    Yoshiyama, Hironori
    Tsurumi, Tatsuya
    Kimura, Hiroshi
    JOURNAL OF VIROLOGY, 2016, 90 (08) : 3873 - 3889
  • [22] Cellular signaling pathways engaged by the Epstein-Barr virus transforming protein LMP1
    Hatzivassiliou, E
    Mosialos, G
    FRONTIERS IN BIOSCIENCE, 2002, 7 : D319 - D329
  • [23] The Epstein-Barr virus encoded LMP1 oncoprotein modulates cell adhesion via regulation of activin A/TGFβ and β1 integrin signalling
    Morris, Mhairi A.
    Dawson, Christopher W.
    Laverick, Louise
    Davis, Alexandra M.
    Dudman, Joe P. R.
    Raveenthiraraj, Sathuwarman
    Ahmad, Zeeshan
    Yap, Lee-Fah
    Young, Lawrence S.
    SCIENTIFIC REPORTS, 2016, 6
  • [24] Epstein-Barr Virus LMP1 oncogene variants in cell lines of different origin
    Yakovleva, L. S.
    Senyuta, N. B.
    Goncharova, E. V.
    Scherback, L. N.
    Smirnova, R. V.
    Pavlish, O. A.
    Gurtsevitch, V. E.
    MOLECULAR BIOLOGY, 2015, 49 (05) : 714 - 722
  • [25] The Epstein-Barr virus encoded LMP1 oncoprotein modulates cell adhesion via regulation of activin A/TGFβ and β1 integrin signalling
    Mhairi A. Morris
    Christopher W. Dawson
    Louise Laverick
    Alexandra M. Davis
    Joe P. R. Dudman
    Sathuwarman Raveenthiraraj
    Zeeshan Ahmad
    Lee-Fah Yap
    Lawrence S. Young
    Scientific Reports, 6
  • [26] Expression of the Epstein-Barr virus protein LMP1 mediates tumor regression in vivo
    Cherney, BW
    Sgadari, C
    Kanegane, C
    Wang, F
    Tosato, G
    BLOOD, 1998, 91 (07) : 2491 - 2500
  • [27] Functional analysis of the mutated Epstein-Barr virus oncoprotein LMP169del:: implications for a new role of naturally occurring LMP1 variants
    Larcher, C
    Bernhard, D
    Schaadt, E
    Adler, B
    Ausserlechner, MJ
    Mitterer, M
    Huemer, HP
    HAEMATOLOGICA, 2003, 88 (12) : 1324 - 1335
  • [28] Transcriptome Changes Induced by Epstein-Barr Virus LMP1 and LMP2A in Transgenic Lymphocytes and Lymphoma
    Shair, Kathy H. Y.
    Raab-Traub, Nancy
    MBIO, 2012, 3 (05):
  • [29] The Epstein-Barr Virus (EBV) Encoded Oncoprotein LMP1 Mediates Down Regulation of Shelterin Proteins, Formation of Telomere Aggregates and Multinuclearity
    Lajoie, Valerie
    Lemieux, Bruno
    Sawan, Bassem
    Lichtensztejn, Daniel
    Lichtensztejn, Zelda
    Rowe, Martin
    Wellinger, Raymund
    Mai, Sabine
    Knecht, Hans
    BLOOD, 2010, 116 (21) : 1492 - 1492
  • [30] EVIDENCE FOR REGULATION OF APOPTOSIS BY LMP1 ONCOPROTEIN OF EPSTEIN-BARR VIRUS IN PATIENTS WITH LOW GRADE B-CELL LEUKEMIC LYMPHOMAS
    Diamantopoulos, P.
    Polonyfi, K.
    Sofotasiou, M.
    Galanopoulos, A.
    Diamantopoulou, G.
    Papadopoulou, V.
    Kalala, F.
    Angelopoulou, M.
    Variami
    Vassilakopoulos, T.
    Siakantaris, M.
    Anagnostopoulos, A.
    Spanakis, N.
    Meletis, J.
    Viniou, N. A.
    HAEMATOLOGICA, 2012, 97 : 647 - 647
12345