Desired and side effects of the supplementation with L-glutamine and L-glutathione in enteric glia of diabetic rats

被引：11

作者：

do Nascimento Bonato Panizzon, Cynthia Priscilla ^{[1
]}

Zanoni, Jacqueline Nelisis ^{[2
]}

Hermes-Uliana, Catchia ^{[3
]}

Trevizan, Aline Rosa ^{[4
]}

Sehaber, Camila Caviquioli ^{[5
]}

Fernandes Pereira, Renata Virginia ^{[2
]}

Linden, David Robert ^{[6
]}

de Miranda Neto, Marcilio Hubner ^{[2
]}

机构：

[1] Fac Integrado Campo Mourao, Campo Mourao, PR, Brazil

[2] Univ Estadual Maringa, Dept Morphol Sci, Maringa, Parana, Brazil

[3] Univ Fed Mato Grosso do Sul, Coxim, MS, Brazil

[4] Univ Estadual Maringa, Dept Clin Anal & Biomed, Maringa, Parana, Brazil

[5] Univ Estadual Maringa, Dept Pharm, Maringa, Parana, Brazil

[6] Mayo Clin, Coll Med, Dept Physiol & Biomed Engn, Rochester, MN USA

来源：

ACTA HISTOCHEMICA | 2016年 / 118卷 / 06期

关键词：

Enteric nervous system; Glia cells; Diabetes mellitus; Antioxidants; V MYENTERIC NEURONS; OXIDATIVE STRESS; MYOSIN-V; GASTROINTESTINAL-TRACT; NERVOUS-SYSTEM; DUODENUM; METABOLISM; PLEXUS; POPULATION; MELLITUS;

D O I：

10.1016/j.acthis.2016.07.008

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Background/Aims: Enteric neuropathy associated with Diabetes Mellitus causes dysfunction in the digestive system, such as: nausea, diarrhea, constipation, vomiting, among others. The aim of this study was to compare the effects of supplementation with 2% L-glutamine and 1% L-glutathione on neurons and enteric glial cells of ileum of diabetic rats. Methods: Thirty male Wistar rats have been used according to these group distributions: Normoglycemic (N), Normoglycemic supplemented with L-glutamine (NG), Normoglycemic supplemented with L-glutathione (NGO), Diabetic (D), Diabetic supplemented with L-glutamine (DG) and Diabetic supplemented with L-glutathione (DGO). After 120 days, the ileum was processed for immunohistochemistry of HuC/D and S100 beta. Quantitative and morphometric analysis have been performed. Results: Diabetic rats presented a decrease in the number of neurons when compared to normoglycemic animals. However, diabetes was not associated with a change in glial density. L-Glutathione prevented the neuronal death in diabetic rats. L-Glutathione increased a glial proliferation in diabetic rats. The neuronal area in diabetic rats increased in relation to the normoglycemics. The diabetic rats supplemented with L-glutamine and L-glutathione showed a smaller neuronal area in comparison to diabetic group. The glial cell area was a decreased in the diabetics. The diabetic rats supplemented with L-glutamine and L-glutathione did not have significant difference in the glial cell body area when compared to diabetic rats. Conclusion: It is concluded that the usage of L-glutamine and L-glutathione as supplements presents both desired and side effects that are different for the same substance in considering normoglycemic or diabetic animals. (C) 2016 Elsevier GmbH. All rights reserved.

引用

页码：625 / 631

页数：7

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