Virtual histopathology with ultraviolet scattering and photoacoustic remote sensing microscopy

被引：9

作者：

Haven, Nathaniel J. M. ^{[1
]}

Martell, Matthew T. ^{[1
]}

Cikaluk, Brendyn D. ^{[1
]}

Restall, Brendon S. ^{[1
]}

McAlister, Ewan ^{[1
]}

Silverman, Sveta ^{[2
]}

Peiris, Lashan ^{[3
]}

Deschenes, Jean ^{[2
]}

Li, Xingyu ^{[1
]}

Zemp, Roger J. ^{[1
]}

机构：

[1] Univ Alberta, iDepartmentof Elect & Comp Engn, 116 St & 85 Ave, Edmonton, AB T6G 2R3, Canada

[2] Univ Alberta, Dept Lab Med & Pathol, 116 St & 85 Ave, Edmonton, AB T6G 2R3, Canada

[3] Univ Alberta, Dept Surg, 116 St & 85 Ave, Edmonton, AB T6G 2R3, Canada

来源：

OPTICS LETTERS | 2021年 / 46卷 / 20期

基金：

加拿大健康研究院; 加拿大自然科学与工程研究理事会;

关键词：

EXCITATION;

D O I：

10.1364/OL.436136

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Realistic label-free virtual histopathology has been a long sought-after goal not yet achieved with current methods. Here, we introduce high-resolution hematoxylin and eosin (H&E)-like virtual histology of unstained human breast lumpectomy specimen sections using ultraviolet scattering-augmented photoacoustic remote sensing microscopy. Together with a colormap-matching algorithm based on blind stain separation from a reference true H&E image, we are able to produce virtual H&E images of unstained tissues with close concordance to true H&E-stained sections, with promising diagnostic utility. (C) 2021 Optical Society of America.

引用

页码：5153 / 5156

页数：4

共 16 条

[1] All-optical Reflection-mode Microscopic Histology of Unstained Human Tissues [J].

Abbasi, Saad ;

Le, Martin ;

Sonier, Bazil ;

Dinakaran, Deepak ;

Bigras, Gilbert ;

Bell, Kevan ;

Mackey, John R. ;

Reza, Parsin Haji .

SCIENTIFIC REPORTS, 2019, 9 (1)

[2]

Bell K., 2020, SCI REP-UK, V10, P1

[3] Single acquisition label-free histology-like imaging with dual-contrast photoacoustic remote sensing microscopy [J].

Ecclestone, Benjamin ;

Dinakaran, Deepak ;

Reza, Parsin Haji .

JOURNAL OF BIOMEDICAL OPTICS, 2021, 26 (05)

[4] Microscopy with ultraviolet surface excitation for rapid slide-free histology [J].

Fereidouni, Farzad ;

Harmany, Zachary T. ;

Tian, Miao ;

Todd, Austin ;

Kintner, John A. ;

McPherson, John D. ;

Borowsky, Alexander D. ;

Bishop, John ;

Lechpammer, Mirna ;

Demos, Stavros G. ;

Levenson, Richard .

NATURE BIOMEDICAL ENGINEERING, 2017, 1 (12) :957-+

[5] Light-sheet microscopy for slide-free non-destructive pathology of large clinical specimens [J].

Glaser, Adam K. ;

Reder, Nicholas P. ;

Chen, Ye ;

McCarty, Erin F. ;

Yin, Chengbo ;

Wei, Linpeng ;

Wang, Yu ;

True, Lawrence D. ;

Liu, Jonathan T. C. .

NATURE BIOMEDICAL ENGINEERING, 2017, 1 (07)

[6] Reflective objective-based ultraviolet photoacoustic remote sensing virtual histopathology [J].

Haven, Nathaniel J. M. ;

Kedarisetti, Pradyumna ;

Restall, Brendon S. ;

Zemp, Roger J. .

OPTICS LETTERS, 2020, 45 (02) :535-538

[7] Ultraviolet photoacoustic remote sensing microscopy [J].

Haven, Nathaniel J. M. ;

Bell, Kevan L. ;

Kedarisetti, Pradyumna ;

Lewis, John D. ;

Zemp, Roger J. .

OPTICS LETTERS, 2019, 44 (14) :3586-3589

[8] F-mode ultraviolet photoacoustic remote sensing for label-free virtual H&E histopathology using a single excitation wavelength [J].

Kedarisetti, Pradyumna ;

Restall, Brendon S. ;

Haven, Nathaniel J. M. ;

Martell, Matthew T. ;

Cikaluk, Brendyn D. ;

Deschenes, Jean ;

Zemp, Roger J. .

OPTICS LETTERS, 2021, 46 (15) :3500-3503

[9] Circular Mixture Modeling of Color Distribution for Blind Stain Separation in Pathology Images [J].

Li, Xingyu ;

Plataniots, Konstantions N. .

IEEE JOURNAL OF BIOMEDICAL AND HEALTH INFORMATICS, 2017, 21 (01) :150-161

[10] A Complete Color Normalization Approach to Histopathology Images Using Color Cues Computed From Saturation-Weighted Statistics [J].

Li, Xingyu ;

Plataniotis, Konstantinos N. .

IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING, 2015, 62 (07) :1862-1873

← 1 2 →