Imaging of hypochlorous acid in mitochondria using an asymmetric near-infrared fluorescent probe with large Stokes shift

被引:56
作者
Hu, Wei [1 ,3 ]
Qiang, Taotao [1 ]
Li, Chenchen [2 ]
Ren, Longfang [1 ]
Cheng, Fei [1 ]
Wang, Baoshuai [1 ]
Li, Mingli [1 ]
Song, Xinjian [2 ]
James, Tony D. [3 ,4 ]
机构
[1] Shaanxi Univ Sci & Technol, Coll Bioresources & Mat Engn, Shaanxi Collaborat Innovat Ctr Ind Auxiliary Chem, Xian 710021, Peoples R China
[2] Hubei Univ Nationalities, Hubei Key Lab Biol Resources Protect & Utilizat, Enshi 445000, Peoples R China
[3] Univ Bath, Dept Chem, Bath BA2 7AY, Avon, England
[4] Henan Normal Univ, Sch Chem & Chem Engn, Xinxiang 453007, Henan, Peoples R China
基金
中国国家自然科学基金;
关键词
Chlorine compounds - Emission spectroscopy - Fluorophores - Infrared devices - Mitochondria - Probes - Signal to noise ratio;
D O I
10.1039/d2sc03833a
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Small-molecule near-infrared (NIR) imaging facilitates deep tissue penetration, low autofluorescence, non-invasive visualization, and a relatively simple operation. As such it has emerged as a popular technique for tracking biological species and events. However, the small Stokes shift of most NIR dyes often results in a low signal-to-noise ratio and self-quenching due to crosstalk between the excitation and emission spectra. With this research, we developed a NIR-based fluorescent probe WD-HOCl for hypochlorous acid (HOCl) detection using the NIR dye TJ730 as the fluorophore, which exhibits a large Stokes shift of 156 nm, with no crosstalk between the excitation and emission spectra. It contains acyl hydrazide as the responsive group and a pyridinium cation as the mitochondria-targeting group. The fluorescence intensity of WD-HOCl was enhanced by 30.1-fold after reacting with HOCl. Imaging studies performed using BV-2 cells indicated that WD-HOCl could be used for endogenous HOCl detection and imaging in living cells exposed to glucose and oxygen deprivation/reperfusion. Finally, we demonstrated that inhibiting the expression of NOX2 reduced the HOCl levels and the severity of oxidative stress during stroke in a mouse model.
引用
收藏
页码:11140 / 11149
页数:10
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