Physical stabilization of DNA-based therapeutics

被引：42
作者：
Anchordoquy, TJ [1 ]
Allison, SD
Molina, MDC
Girouard, LG
Carson, TK
机构：
[1] Univ Colorado, Sch Pharm, Denver, CO 80262 USA
[2] PR Pharmaceut, Ft Collins, CO USA
来源：
DRUG DISCOVERY TODAY | 2001年 / 6卷 / 09期
关键词：
D O I：
10.1016/S1359-6446(01)01739-1
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
The development of non-viral vectors for gene delivery has primarily focused on improving the efficiency of gene transfer in vivo. Although there is clearly a need to improve delivery efficiency, studies also indicate that the physical stability of non-viral vectors is not nearly adequate for a marketable pharmaceutical product. Here, we describe the different strategies that have been used to enhance stability and discuss the mechanisms by which prolonged stabilization (>2 years) might be achieved.
引用
收藏
页码：463 / 470
页数：8
相关论文
共 71 条
[11]   PLGA microspheres containing plasmid DNA: Preservation of supercoiled DNA via cryopreparation and carbohydrate stabilization [J].
Ando, S ;
Putnam, D ;
Pack, DW ;
Langer, R .
JOURNAL OF PHARMACEUTICAL SCIENCES, 1999, 88 (01) :126-130
[12]  
[Anonymous], [No title captured]
[13]   STABILIZATION OF PROTEIN-STRUCTURE BY SUGARS [J].
ARAKAWA, T ;
TIMASHEFF, SN .
BIOCHEMISTRY, 1982, 21 (25) :6536-6544
[14]   Gene therapy for cancer [J].
Blaese, RM .
SCIENTIFIC AMERICAN, 1997, 276 (06) :111-115
[15]   REACTIONS OF OXYL RADICALS WITH DNA [J].
BREEN, AP ;
MURPHY, JA .
FREE RADICAL BIOLOGY AND MEDICINE, 1995, 18 (06) :1033-1077
[16]   LIPOSOME-MEDIATED CFTR GENE-TRANSFER TO THE NASAL EPITHELIUM OF PATIENTS WITH CYSTIC-FIBROSIS [J].
CAPLEN, NJ ;
ALTON, EWFW ;
MIDDLETON, PG ;
DORIN, JR ;
STEVENSON, BJ ;
GAO, X ;
DURHAM, SR ;
JEFFERY, PK ;
HODSON, ME ;
COUTELLE, C ;
HUANG, L ;
PORTEOUS, DJ ;
WILLIAMSON, R ;
GEDDES, DM .
NATURE MEDICINE, 1995, 1 (01) :39-46
[17]   THE MECHANISM OF CRYOPROTECTION OF PROTEINS BY SOLUTES [J].
CARPENTER, JF ;
CROWE, JH .
CRYOBIOLOGY, 1988, 25 (03) :244-255
[18]   AN INFRARED SPECTROSCOPIC STUDY OF THE INTERACTIONS OF CARBOHYDRATES WITH DRIED PROTEINS [J].
CARPENTER, JF ;
CROWE, JH .
BIOCHEMISTRY, 1989, 28 (09) :3916-3922
[19]   Gene therapy of human severe combined immunodeficiency (SCID)-X1 disease [J].
Cavazzana-Calvo, M ;
Hacein-Bey, S ;
Basile, CD ;
Gross, F ;
Yvon, E ;
Nusbaum, P ;
Selz, F ;
Hue, C ;
Certain, S ;
Casanova, JL ;
Bousso, P ;
Le Deist, F ;
Fischer, A .
SCIENCE, 2000, 288 (5466) :669-672
[20]   USE OF SUBAMBIENT THERMAL-ANALYSIS TO OPTIMIZE PROTEIN LYOPHILIZATION [J].
CHANG, BS ;
RANDALL, CS .
CRYOBIOLOGY, 1992, 29 (05) :632-656
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