Testing DNA barcodes in closely related species of Curcuma (Zingiberaceae) from Myanmar and China
被引:67
作者:
Chen, Juan
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Chinese Acad Sci, Key Lab Plant Resources Conservat & Sustainable U, South China Bot Garden, Guangzhou 510650, Guangdong, Peoples R ChinaChinese Acad Sci, Key Lab Plant Resources Conservat & Sustainable U, South China Bot Garden, Guangzhou 510650, Guangdong, Peoples R China
Chen, Juan
[1
]
Zhao, Jietang
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机构:
South China Agr Univ, Coll Hort, Guangzhou 510642, Guangdong, Peoples R ChinaChinese Acad Sci, Key Lab Plant Resources Conservat & Sustainable U, South China Bot Garden, Guangzhou 510650, Guangdong, Peoples R China
Zhao, Jietang
[2
]
Erickson, David L.
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机构:
Smithsonian Inst, Dept Bot, Natl Museum Nat Hist, MRC 166, Washington, DC 20013 USAChinese Acad Sci, Key Lab Plant Resources Conservat & Sustainable U, South China Bot Garden, Guangzhou 510650, Guangdong, Peoples R China
Erickson, David L.
[3
]
Xia, Nianhe
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机构:
Chinese Acad Sci, Key Lab Plant Resources Conservat & Sustainable U, South China Bot Garden, Guangzhou 510650, Guangdong, Peoples R ChinaChinese Acad Sci, Key Lab Plant Resources Conservat & Sustainable U, South China Bot Garden, Guangzhou 510650, Guangdong, Peoples R China
Xia, Nianhe
[1
]
Kress, W. John
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机构:
Smithsonian Inst, Dept Bot, Natl Museum Nat Hist, MRC 166, Washington, DC 20013 USAChinese Acad Sci, Key Lab Plant Resources Conservat & Sustainable U, South China Bot Garden, Guangzhou 510650, Guangdong, Peoples R China
Kress, W. John
[3
]
机构:
[1] Chinese Acad Sci, Key Lab Plant Resources Conservat & Sustainable U, South China Bot Garden, Guangzhou 510650, Guangdong, Peoples R China
[2] South China Agr Univ, Coll Hort, Guangzhou 510642, Guangdong, Peoples R China
[3] Smithsonian Inst, Dept Bot, Natl Museum Nat Hist, MRC 166, Washington, DC 20013 USA
chloroplast region;
DNA barcoding;
ITS2;
species identification;
CHROMOSOME-NUMBERS;
LAND PLANTS;
IDENTIFICATION;
L;
PHYLOGENY;
DIVERGENCE;
MORPHOLOGY;
SIZE;
D O I:
10.1111/1755-0998.12319
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
The genus Curcuma L. is commonly used as spices, medicines, dyes and ornamentals. Owing to its economic significance and lack of clear-cut morphological differences between species, this genus is an ideal case for developing DNA barcodes. In this study, four chloroplast DNA regions (matK, rbcL, trnH-psbA and trnL-F) and one nuclear region (ITS2) were generated for 44 Curcuma species and five species from closely related genera, represented by 96 samples. PCR amplification success rate, intra- and inter-specific genetic distance variation and the correct identification percentage were taken into account to assess candidate barcode regions. PCR and sequence success rate were high in matK (89.7%), rbcL (100%), trnH-psbA (100%), trnL-F (95.7%) and ITS2 (82.6%) regions. The results further showed that four candidate chloroplast barcoding regions (matK, rbcL, trnH-psbA and trnL-F) yield no barcode gaps, indicating that the genus Curcuma represents a challenging group for DNA barcoding. The ITS2 region presented large interspecific variation and provided the highest correct identification rates (46.7%) based on BLASTClust method among the five regions. However, the ITS2 only provided 7.9% based on NJ tree method. An increase in discriminatory power needs the development of more variable markers.
机构:
Chinese Acad Sci, South China Bot Garden, Key Lab Plant Resources Conservat & Sustainable U, CN-510650 Guangzhou, Guangdong, Peoples R ChinaChinese Acad Sci, South China Bot Garden, Key Lab Plant Resources Conservat & Sustainable U, CN-510650 Guangzhou, Guangdong, Peoples R China
Chen, Juan
;
Xia, Nian-He
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机构:
Chinese Acad Sci, South China Bot Garden, Key Lab Plant Resources Conservat & Sustainable U, CN-510650 Guangzhou, Guangdong, Peoples R ChinaChinese Acad Sci, South China Bot Garden, Key Lab Plant Resources Conservat & Sustainable U, CN-510650 Guangzhou, Guangdong, Peoples R China
机构:
Chinese Acad Sci, Key Lab Plant Resources Conservat & Sustainable U, South China Bot Garden, Guangzhou 510650, Guangdong, Peoples R ChinaChinese Acad Sci, Key Lab Plant Resources Conservat & Sustainable U, South China Bot Garden, Guangzhou 510650, Guangdong, Peoples R China
Chen, Juan
;
Xia, Nianhe
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h-index: 0
机构:
Chinese Acad Sci, Key Lab Plant Resources Conservat & Sustainable U, South China Bot Garden, Guangzhou 510650, Guangdong, Peoples R ChinaChinese Acad Sci, Key Lab Plant Resources Conservat & Sustainable U, South China Bot Garden, Guangzhou 510650, Guangdong, Peoples R China
Xia, Nianhe
;
Zhao, Jietang
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h-index: 0
机构:
Univ Florida, IFAS, Midflorida Res & Educ Ctr, Apopka, FL 32703 USAChinese Acad Sci, Key Lab Plant Resources Conservat & Sustainable U, South China Bot Garden, Guangzhou 510650, Guangdong, Peoples R China
Zhao, Jietang
;
Chen, Jianjun
论文数: 0引用数: 0
h-index: 0
机构:
Univ Florida, IFAS, Midflorida Res & Educ Ctr, Apopka, FL 32703 USAChinese Acad Sci, Key Lab Plant Resources Conservat & Sustainable U, South China Bot Garden, Guangzhou 510650, Guangdong, Peoples R China
Chen, Jianjun
;
Henny, Richard J.
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h-index: 0
机构:
Univ Florida, IFAS, Midflorida Res & Educ Ctr, Apopka, FL 32703 USAChinese Acad Sci, Key Lab Plant Resources Conservat & Sustainable U, South China Bot Garden, Guangzhou 510650, Guangdong, Peoples R China
机构:
Chinese Acad Sci, S China Bot Garden, Key Lab Plant Resources Conservat & Sustainable U, Guangzhou 510650, Guangdong, Peoples R China
Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R ChinaChinese Acad Sci, S China Bot Garden, Key Lab Plant Resources Conservat & Sustainable U, Guangzhou 510650, Guangdong, Peoples R China
Chen, Juan
;
Xia, Nian-He
论文数: 0引用数: 0
h-index: 0
机构:
Chinese Acad Sci, S China Bot Garden, Key Lab Plant Resources Conservat & Sustainable U, Guangzhou 510650, Guangdong, Peoples R ChinaChinese Acad Sci, S China Bot Garden, Key Lab Plant Resources Conservat & Sustainable U, Guangzhou 510650, Guangdong, Peoples R China
机构:
Chinese Acad Sci, S China Bot Garden, Key Lab Plant Resources Conservat & Sustainable U, CN-510650 Guangzhou, Guangdong, Peoples R China
Chinese Acad Sci, Grad Univ, CN-100049 Beijing, Peoples R ChinaChinese Acad Sci, S China Bot Garden, Key Lab Plant Resources Conservat & Sustainable U, CN-510650 Guangzhou, Guangdong, Peoples R China
Chen, Juan
;
Xia, Nian-He
论文数: 0引用数: 0
h-index: 0
机构:
Chinese Acad Sci, S China Bot Garden, Key Lab Plant Resources Conservat & Sustainable U, CN-510650 Guangzhou, Guangdong, Peoples R ChinaChinese Acad Sci, S China Bot Garden, Key Lab Plant Resources Conservat & Sustainable U, CN-510650 Guangzhou, Guangdong, Peoples R China
机构:
Chinese Acad Sci, South China Bot Garden, Key Lab Plant Resources Conservat & Sustainable U, CN-510650 Guangzhou, Guangdong, Peoples R ChinaChinese Acad Sci, South China Bot Garden, Key Lab Plant Resources Conservat & Sustainable U, CN-510650 Guangzhou, Guangdong, Peoples R China
Chen, Juan
;
Xia, Nian-He
论文数: 0引用数: 0
h-index: 0
机构:
Chinese Acad Sci, South China Bot Garden, Key Lab Plant Resources Conservat & Sustainable U, CN-510650 Guangzhou, Guangdong, Peoples R ChinaChinese Acad Sci, South China Bot Garden, Key Lab Plant Resources Conservat & Sustainable U, CN-510650 Guangzhou, Guangdong, Peoples R China
机构:
Chinese Acad Sci, Key Lab Plant Resources Conservat & Sustainable U, South China Bot Garden, Guangzhou 510650, Guangdong, Peoples R ChinaChinese Acad Sci, Key Lab Plant Resources Conservat & Sustainable U, South China Bot Garden, Guangzhou 510650, Guangdong, Peoples R China
Chen, Juan
;
Xia, Nianhe
论文数: 0引用数: 0
h-index: 0
机构:
Chinese Acad Sci, Key Lab Plant Resources Conservat & Sustainable U, South China Bot Garden, Guangzhou 510650, Guangdong, Peoples R ChinaChinese Acad Sci, Key Lab Plant Resources Conservat & Sustainable U, South China Bot Garden, Guangzhou 510650, Guangdong, Peoples R China
Xia, Nianhe
;
Zhao, Jietang
论文数: 0引用数: 0
h-index: 0
机构:
Univ Florida, IFAS, Midflorida Res & Educ Ctr, Apopka, FL 32703 USAChinese Acad Sci, Key Lab Plant Resources Conservat & Sustainable U, South China Bot Garden, Guangzhou 510650, Guangdong, Peoples R China
Zhao, Jietang
;
Chen, Jianjun
论文数: 0引用数: 0
h-index: 0
机构:
Univ Florida, IFAS, Midflorida Res & Educ Ctr, Apopka, FL 32703 USAChinese Acad Sci, Key Lab Plant Resources Conservat & Sustainable U, South China Bot Garden, Guangzhou 510650, Guangdong, Peoples R China
Chen, Jianjun
;
Henny, Richard J.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Florida, IFAS, Midflorida Res & Educ Ctr, Apopka, FL 32703 USAChinese Acad Sci, Key Lab Plant Resources Conservat & Sustainable U, South China Bot Garden, Guangzhou 510650, Guangdong, Peoples R China
机构:
Chinese Acad Sci, S China Bot Garden, Key Lab Plant Resources Conservat & Sustainable U, Guangzhou 510650, Guangdong, Peoples R China
Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R ChinaChinese Acad Sci, S China Bot Garden, Key Lab Plant Resources Conservat & Sustainable U, Guangzhou 510650, Guangdong, Peoples R China
Chen, Juan
;
Xia, Nian-He
论文数: 0引用数: 0
h-index: 0
机构:
Chinese Acad Sci, S China Bot Garden, Key Lab Plant Resources Conservat & Sustainable U, Guangzhou 510650, Guangdong, Peoples R ChinaChinese Acad Sci, S China Bot Garden, Key Lab Plant Resources Conservat & Sustainable U, Guangzhou 510650, Guangdong, Peoples R China
机构:
Chinese Acad Sci, S China Bot Garden, Key Lab Plant Resources Conservat & Sustainable U, CN-510650 Guangzhou, Guangdong, Peoples R China
Chinese Acad Sci, Grad Univ, CN-100049 Beijing, Peoples R ChinaChinese Acad Sci, S China Bot Garden, Key Lab Plant Resources Conservat & Sustainable U, CN-510650 Guangzhou, Guangdong, Peoples R China
Chen, Juan
;
Xia, Nian-He
论文数: 0引用数: 0
h-index: 0
机构:
Chinese Acad Sci, S China Bot Garden, Key Lab Plant Resources Conservat & Sustainable U, CN-510650 Guangzhou, Guangdong, Peoples R ChinaChinese Acad Sci, S China Bot Garden, Key Lab Plant Resources Conservat & Sustainable U, CN-510650 Guangzhou, Guangdong, Peoples R China