Testing DNA barcodes in closely related species of Curcuma (Zingiberaceae) from Myanmar and China

被引:67
作者
Chen, Juan [1 ]
Zhao, Jietang [2 ]
Erickson, David L. [3 ]
Xia, Nianhe [1 ]
Kress, W. John [3 ]
机构
[1] Chinese Acad Sci, Key Lab Plant Resources Conservat & Sustainable U, South China Bot Garden, Guangzhou 510650, Guangdong, Peoples R China
[2] South China Agr Univ, Coll Hort, Guangzhou 510642, Guangdong, Peoples R China
[3] Smithsonian Inst, Dept Bot, Natl Museum Nat Hist, MRC 166, Washington, DC 20013 USA
基金
中国国家自然科学基金;
关键词
chloroplast region; DNA barcoding; ITS2; species identification; CHROMOSOME-NUMBERS; LAND PLANTS; IDENTIFICATION; L; PHYLOGENY; DIVERGENCE; MORPHOLOGY; SIZE;
D O I
10.1111/1755-0998.12319
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The genus Curcuma L. is commonly used as spices, medicines, dyes and ornamentals. Owing to its economic significance and lack of clear-cut morphological differences between species, this genus is an ideal case for developing DNA barcodes. In this study, four chloroplast DNA regions (matK, rbcL, trnH-psbA and trnL-F) and one nuclear region (ITS2) were generated for 44 Curcuma species and five species from closely related genera, represented by 96 samples. PCR amplification success rate, intra- and inter-specific genetic distance variation and the correct identification percentage were taken into account to assess candidate barcode regions. PCR and sequence success rate were high in matK (89.7%), rbcL (100%), trnH-psbA (100%), trnL-F (95.7%) and ITS2 (82.6%) regions. The results further showed that four candidate chloroplast barcoding regions (matK, rbcL, trnH-psbA and trnL-F) yield no barcode gaps, indicating that the genus Curcuma represents a challenging group for DNA barcoding. The ITS2 region presented large interspecific variation and provided the highest correct identification rates (46.7%) based on BLASTClust method among the five regions. However, the ITS2 only provided 7.9% based on NJ tree method. An increase in discriminatory power needs the development of more variable markers.
引用
收藏
页码:337 / 348
页数:12
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