A protective and broadly cross-neutralizing epitope of human papillomavirus L2

被引：185

作者：

Gambhira, Ratish ^{[1
]}

Karanam, Balasubramanyam ^{[1
]}

Jagu, Subhashini ^{[1
]}

Roberts, Jeffrey N. ^{[4
]}

Buck, Christopher B. ^{[4
]}

Bossis, Ioannis ^{[1
]}

Alphs, Hannah ^{[1
]}

Culp, Timothy ^{[5
]}

Christensen, Neil D. ^{[5
]}

Roden, Richard B. S. ^{[1
,2
,3
]}

机构：

[1] Johns Hopkins Univ, Sch Med, Dept Pathol, Baltimore, MD 21231 USA

[2] Johns Hopkins Univ, Sch Med, Dept Oncol, Baltimore, MD 21231 USA

[3] Johns Hopkins Univ, Sch Med, Dept Gynecol & Obstet, Baltimore, MD 21231 USA

[4] NCI, Cellular Oncol Lab, Bethesda, MD 20892 USA

[5] Penn State Univ, Coll Med, Dept Microbiol & Immunol, Hershey, PA USA

来源：

JOURNAL OF VIROLOGY | 2007年 / 81卷 / 24期

关键词：

D O I：

10.1128/JVI.00936-07

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We generated a monoclonal antibody, RG-1, that binds to highly conserved L2 residues 17 to 36 and neutralizes human papillomavirus 16 (HPV16) and HPV18. Passive immunotherapy with RG-1 was protective in mice. Antiserum to the HPV16 L2 peptide comprising residues 17 to 36 (peptide 17-36) neutralized pseudoviruses HPV5, HPV6, HPV16, HPV 18, HPV31, HPV 45, HPV 52, HPV 58, bovine papillomavirus 1, and HPV11 native virions. Depletion of HPV16 L2 peptide 17-36-reactive antibodies from cross-neutralizing rabbit and human L2-specific sera abolished cross-neutralization and drastically reduced neutralization of the cognate type. This cross-neutralization of diverse HPVs associated with cervical cancer, genital warts, and epidermodysplasia verruciformis suggests the possibility of a broadly protective, peptide-based vaccine.

引用

页码：13927 / 13931

页数：5

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