A novel non-contact communication between human keratinocytes and T cells: Exosomes derived from keratinocytes support superantigen-induced proliferation of resting T cells

被引：28

作者：

Cai, Xiao-Wei ^{[1
]}

Zhu, Rong ^{[1
]}

Ran, Lei ^{[1
]}

Li, Yi-Qian ^{[1
]}

Huang, Ke ^{[1
]}

Peng, Jing ^{[1
]}

He, Wei ^{[1
]}

Zhou, Chun-Li ^{[1
]}

Wang, Ru-Peng ^{[1
]}

机构：

[1] Third Mil Med Univ, Xinqiao Hosp, Dept Dermatol & Rheumatol Immunol, 108 Xinqiao St, Chongqing 400000, Peoples R China

来源：

MOLECULAR MEDICINE REPORTS | 2017年 / 16卷 / 05期

基金：

中国国家自然科学基金;

关键词：

exosomes; T cells; keratinocytes; Staphylococcal aureus enterotoxin B; Staphylococcus aureus; ATOPIC-DERMATITIS; DENDRITIC CELLS; COLONIZATION; INDUCTION; PSORIASIS; ADHESION; ROLES;

D O I：

10.3892/mmr.2017.7492

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

It is widely accepted that keratinocytes act as non-professional antigen-presenting cells and support superantigen-induced proliferation of resting T cells; however, it remains unknown whether keratinocytes function in situ with T cells via a non-contact mechanism. The current study used a transwell co-culture system and demonstrated, for the first time to the best of the authors' knowledge, that HaCaT cells (the human keratinocyte cell line) did induce T cell proliferation via indirect contact. The data further indicated that exosomes, small membrane vesicles that transfer antigens to recipient cells, are also involved in the superantigen-associated immunity of keratinocytes. The current study provided experimental evidence that HaCaT-exosomes contained MHC I and II, and could interact with T cells. In addition, following interferon. stimulation, Staphylococcal aureus enterotoxin B-loaded HaCaT cells secreted exosomes to induce the proliferation of CD4+ and CD8+ T cells in vitro. This novel biological function of exosomes reveals a new mechanism of how keratinocytes participate in bacterial superantigen-induced immune responses.

引用

页码：7032 / 7038

页数：7

共 34 条

[1]

Abeck D, 1998, BRIT J DERMATOL, V139, P13

[2] Exosomes as potent cell-free peptide-based vaccine.: I.: Dendritic cell-derived exosomes transfer functional MHC class I/peptide complexes to dendritic cells [J].