Bacterial virulence against an oceanic bloom-forming phytoplankter is mediated by algal DMSP

被引:72
作者
Barak-Gavish, Noa [1 ]
Frada, Miguel Jose [1 ,2 ,3 ]
Ku, Chuan [1 ]
Lee, Peter A. [4 ]
DiTullio, Giacomo R. [4 ]
Malitsky, Sergey [1 ,5 ]
Aharoni, Asaph [1 ]
Green, Stefan J. [6 ]
Rotkopf, Ron [5 ]
Kartvelishvily, Elena [7 ]
Sheyn, Uri [1 ]
Schatz, Daniella [1 ]
Vardi, Assaf [1 ]
机构
[1] Weizmann Inst Sci, Dept Plant & Environm Sci, IL-7610001 Rehovot, Israel
[2] Interuniv Inst Marine Sci, IL-88103 Elat, Israel
[3] Hebrew Univ Jerusalem, Silberman Inst Life Sci, Dept Ecol Evolut & Behav, IL-9190401 Jerusalem, Israel
[4] Coll Charleston, Hollings Marine Lab, Charleston, SC 29412 USA
[5] Weizmann Inst Sci, Dept Biol Serv, IL-7610001 Rehovot, Israel
[6] Univ Illinois, DNA Serv Facil, Chicago, IL 60612 USA
[7] Weizmann Inst Sci, Dept Chem Res Support, IL-7610001 Rehovot, Israel
来源
SCIENCE ADVANCES | 2018年 / 4卷 / 10期
基金
欧洲研究理事会; 美国国家科学基金会;
关键词
VOLATILE SULFUR-COMPOUNDS; EMILIANIA-HUXLEYI; ATMOSPHERIC SULFUR; MARINE-BACTERIA; DIMETHYLSULFONIOPROPIONATE; VIRUS; COCCOLITHOPHORE; DIVERSITY; ALIGNMENT; DYNAMICS;
D O I
10.1126/sciadv.aau5716
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Emiliania huxleyi is a bloom-forming microalga that affects the global sulfur cycle by producing large amounts of dimethylsulfoniopropionate (DMSP) and its volatile metabolic product dimethyl sulfide. Top-down regulation of E. huxleyi blooms has been attributed to viruses and grazers; however, the possible involvement of algicidal bacteria in bloom demise has remained elusive. We demonstrate that a Roseobacter strain, Sulfitobacter D7, that we isolated from a North Atlantic E. huxleyi bloom, exhibited algicidal effects against E. huxleyi upon coculturing. Both the alga and the bacterium were found to co-occur during a natural E. huxleyi bloom, therefore establishing this host-pathogen system as an attractive, ecologically relevant model for studying algal-bacterial interactions in the oceans. During interaction, Sulfitobacter D7 consumed and metabolized algal DMSP to produce high amounts of methanethiol, an alternative product of DMSP catabolism. We revealed a unique strain-specific response, in which E. huxleyi strains that exuded higher amounts of DMSP were more susceptible to Sulfitobacter D7 infection. Intriguingly, exogenous application of DMSP enhanced bacterial virulence and induced susceptibility in an algal strain typically resistant to the bacterial pathogen. This enhanced virulence was highly specific to DMSP compared to addition of propionate and glycerol which had no effect on bacterial virulence. We propose a novel function for DMSP, in addition to its central role in mutualistic interactions among marine organisms, as a mediator of bacterial virulence that may regulate E. huxleyi blooms.
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页数:12
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