An effective combined-enhancer for the PCR amplification of GC-rich longer DNA sequences

DNA manipulation technology is facing more challenges in the postgenomics era. The amplification of GC-rich longer DNA fragments PCR more difficult than others. through more and more enhancers have been investigated for their potential implications in developing better gene technology. in this study, we reported a beneficial effect of combined-enhancer in enhancing the specificity and total efficiency of GC-rich (GC%>= 65%) longer DNA fragments PCR. Combined-enhancer is a concentration-dependent combination of 5.5M betaine,0.5u pfu enzyme, 1.5mM the mixture of 1,2-propylene glycol and ethylene glycol (the ration of 1,2-propylene glycol and ethylene glycol = 1:1)in 25 mu L PCR condition. The combined-enhancer broadly enhanced the quantitative and/or qualitative output of GC-rich ( GC%>= 65) longer DNA fragments PCRs.