The Application of Phenotypic High-Throughput Screening Techniques to Cardiovascular Research

被引：14

作者：

Etzion, Yoram ^{[1
]}

Muslin, Anthony J. ^{[1
]}

机构：

[1] Washington Univ, Sch Med, Cardiovasc Res Ctr, St Louis, MO 63110 USA

来源：

TRENDS IN CARDIOVASCULAR MEDICINE | 2009年 / 19卷 / 06期

关键词：

SMALL-MOLECULE INHIBITOR; CASSETTE TRANSPORTER A1; CHEMICAL GENETICS; CARDIOMYOGENESIS; IDENTIFICATION; ASSAY;

D O I：

10.1016/j.tcm.2009.12.006

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

In traditional pure protein high-throughput drug screens, also called in vitro screens, individual compounds from a small molecule collection are tested to determine whether they inhibit the enzymatic activity or binding properties of a purified target protein. In contrast, phenotypic high-throughput drug screens, also called chemical genetic or in vivo screens, investigate the ability of individual compounds from a collection to inhibit a biological process or disease model in live cells or intact organisms. In this review, the role of phenotypic screening techniques to identify novel therapeutic agents for the treatment of cardiovascular disease will be discussed. (Trends Cardiovasc Med 2009;19:207-212) (C) 2009, Elsevier Inc.

引用

页码：207 / 212

页数：6

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