Quantitative mass spectrometry identifies insulin signaling targets in C-elegans

被引：246

作者：

Dong, Meng-Qiu

Venable, John D.

Au, Nora

Xu, Tao

Park, Sung Kyu

Cociorva, Daniel

Johnson, Jeffrey R.

Dillin, Andrew

Yates, John R., III ^{[1
]}

机构：

[1] Scripps Res Inst, La Jolla, CA 92037 USA

[2] Salk Inst Biol Studies, La Jolla, CA 92037 USA

[3] Univ Calif San Diego, La Jolla, CA 92093 USA

来源：

SCIENCE | 2007年 / 317卷 / 5838期

关键词：

D O I：

10.1126/science.1139952

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

DAF-2, an insulin receptor-like protein, regulates metabolism, development, and aging in Caenorhabditis elegans. In a quantitative proteomic study, we identified 86 proteins that were more or less abundant in long-lived daf-2 mutant worms than in wild-type worms. Genetic studies on a subset of these proteins indicated that they act in one or more processes regulated by DAF-2, including entry into the dauer developmental stage and aging. In particular, we discovered a compensatory mechanism activated in response to reduced DAF-2 signaling, which involves the protein phosphatase calcineurin.

引用

收藏

页码：660 / 663

页数：4

相关论文

共 21 条

[1] Calcineurin, a calcium/calmodulin-dependent protein phosphatase, is involved in movement, fertility, egg laying, and growth in Caenorhabditis elegans [J].

Bandyopadhyay, J ;

Lee, J ;

Lee, J ;

Lee, JI ;

Yu, JR ;

Jee, C ;

Cho, JH ;

Jung, S ;

Lee, MH ;

Zannoni, S ;

Singson, A ;

Kim, DH ;

Koo, HS ;

Ahnn, J .

MOLECULAR BIOLOGY OF THE CELL, 2002, 13 (09) :3281-3293

[2] Timing requirements for insulin/IGF-1 signaling in C-elegans [J].

Dillin, A ;

Crawford, DK ;

Kenyon, C .

SCIENCE, 2002, 298 (5594) :830-834

[3]

Gems D, 1998, GENETICS, V150, P129

[4] New genes tied to endocrine, metabolic, and dietary regulation of lifespan from a Caenorhabditis elegans genomic RNAi screen [J].

Hansen, M ;

Hsu, AL ;

Dillin, A ;

Kenyon, C .

PLOS GENETICS, 2005, 1 (01) :119-128

[5] The daf-2 gene network for longevity regulates oxidative stress resistance and Mn-superoxide dismutase gene expression in Caenorhabditis elegans [J].

Honda, Y ;

Honda, S .

FASEB JOURNAL, 1999, 13 (11) :1385-1393

[6] A C-ELEGANS MUTANT THAT LIVES TWICE AS LONG AS WILD-TYPE [J].

KENYON, C ;

CHANG, J ;

GENSCH, E ;

RUDNER, A ;

TABTIANG, R .

NATURE, 1993, 366 (6454) :461-464

[7] daf-2, an insulin receptor-like gene that regulates longevity and diapause in Caenorhabditis elegans [J].

Kimura, KD ;

Tissenbaum, HA ;

Liu, YX ;

Ruvkun, G .

SCIENCE, 1997, 277 (5328) :942-946

[8] Metabolic labeling of C-elegans and D-melanogaster for quantitative proteomics [J].

Krijgsveld, J ;

Ketting, RF ;

Mahmoudi, T ;

Johansen, J ;

Artal-Sanz, M ;

Verrijzer, CP ;

Plasterk, RHA ;

Heck, AJR .

NATURE BIOTECHNOLOGY, 2003, 21 (08) :927-931

[9] Negative regulation and gain control of sensory neurons by the C-elegans calcineurin TAX-6 [J].

Kuhara, A ;

Inada, H ;

Katsura, I ;

Mori, I .

NEURON, 2002, 33 (05) :751-763

[10] Opposing functions of calcineurin and CaMKII regulate G-protein signaling in egg-laying behavior of C-elegans [J].

Lee, J ;

Jee, C ;

Song, HO ;

Bandyopadhyay, J ;

Lee, JI ;

Yu, JR ;

Lee, J ;

Park, BJ ;

Ahnn, J .

JOURNAL OF MOLECULAR BIOLOGY, 2004, 344 (02) :585-595