Molecular Characterization of Mycobiota and Aflatoxin Contamination of Retail Wheat Flours from Jeddah Markets

被引：30
作者：
Gashgari, Rukaia M. [2 ]
Shebany, Yassmin M. [3 ]
Gherbawy, Youssuf A. [1 ]
机构：
[1] Taif Univ, Dept Biol Sci, Fac Sci, At Taif, Saudi Arabia
[2] King Abdulaziz Univ, Girls Coll, Jeddah 21413, Saudi Arabia
[3] S Valley Univ, Fac Sci, Dept Bot, Qena, Egypt
来源：
FOODBORNE PATHOGENS AND DISEASE | 2010年 / 7卷 / 09期
关键词：
POLYMERASE-CHAIN-REACTION; A PRODUCING STRAINS; ASPERGILLUS-FLAVUS; NATURAL OCCURRENCE; GENETIC DIVERSITY; PCR; MYCOFLORA; PENICILLIUM; PARASITICUS; QUANTIFICATION;
D O I：
10.1089/fpd.2009.0506
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
The mycological profile of the retail wheat flour selling in different markets at Jeddah (Kingdom of Saudi Arabia) was studied. The most common genera were Aspergillus (isolated from 70% of the tested samples), Penicillium (30%), Eurotium (14%), and in a lesser extent Fusarium (20%) and Alternaria (18%). Twenty-nine strains of Aspergillus flavus were screened for their ability to produce aflatoxins (AFs). Four strains produced only aflatoxin B1 (AFB1), two strains produced AFB1 and aflatoxin B2, and one strain produced AFB1, aflatoxin G1, and aflatoxin G2. Random amplified polymorphic DNA-polymerase chain reaction technique could not differentiate between toxigenic and nontoxigenic strains of A. flavus. AF regulatory gene was detected in three flour samples
引用
收藏
页码：1047 / 1054
页数：8
相关论文
共 90 条
[1]   Survey of fungal counts and natural occurrence of aflatoxins in Malaysian starch-based foods [J].
Abdullah, N ;
Nawawi, A ;
Othman, I .
MYCOPATHOLOGIA, 1998, 143 (01) :53-58
[2]   New PCR method to differentiate species in the Aspergillus niger aggregate [J].
Accensi, F ;
Cano, J ;
Figuera, L ;
Abarca, ML ;
Cabañes, FJ .
FEMS MICROBIOLOGY LETTERS, 1999, 180 (02) :191-196
[3]   Associated field mycobiota on malt barley [J].
Andersen, B ;
Thrane, U ;
Svendsen, A ;
Rasmussen, IA .
CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE, 1996, 74 (06) :854-858
[4]  
*AOAC, 1980, OFF METH AN, P429
[5]  
Aydin A, 2008, J FOOD DRUG ANAL, V16, P48
[6]  
Baliukoniene V, 2003, ANN AGR ENV MED, V10, P223
[7]   GENETIC DIVERSITY IN ASPERGILLUS-FLAVUS - ASSOCIATION WITH AFLATOXIN PRODUCTION AND MORPHOLOGY [J].
BAYMAN, P ;
COTTY, PJ .
CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE, 1993, 71 (01) :23-31
[8]   Mycobiota and mycotoxins in fermented feed, wheat grains and corn grains in Southeastern Buenos Aires Province, Argentina [J].
Beatriz Roige, Marcela ;
Mariela Aranguren, Sandra ;
Riccio, Maria Belen ;
Pereyra, Silvia ;
Luis Soraci, Alejandro ;
Ofelia Tapia, Maria .
REVISTA IBEROAMERICANA DE MICOLOGIA, 2009, 26 (04) :233-237
[9]  
BENNETT JW, 1994, FEMS SYMP, P51
[10]   Microbiology of wheat and flour milling in Australia [J].
Berghofer, LK ;
Hocking, AD ;
Miskelly, D ;
Jansson, E .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2003, 85 (1-2) :137-149
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