GH, IGF-I and GH receptors mRNA expression in response to growth impairment following a food deprivation period in individually housed cichlid fish Cichlasoma dimerus

被引:15
作者
Horacio Delgadin, Tomas [1 ,2 ]
Perez Sirkin, Daniela Irina [1 ,2 ]
Paula Di Yorio, Maria [1 ,2 ]
Eda Arranz, Silvia [3 ,4 ]
Gabriela Vissio, Paula [1 ,2 ]
机构
[1] FCEN UBA, DBBE, Lab Neuroendocrinol Crecimiento & Reprod, Buenos Aires, DF, Argentina
[2] IBBEA CONICET UBA, Buenos Aires, DF, Argentina
[3] Consejo Nacl Invest Cient & Tecn, Fac Ciencias Bioquim & Farmaceut, Area Biol, Inst Biol Mol & Celular Rosario IBR CONICET, RA-2000 Rosario, Santa Fe, Argentina
[4] Univ Nacl Rosario, RA-2000 Rosario, Santa Fe, Argentina
关键词
Growth hormone; GHR1; GHR2; IGF-I; Cichlid; TISSUE-SPECIFIC EXPRESSION; HORMONE-RECEPTOR; RAINBOW-TROUT; MOLECULAR CHARACTERIZATION; SOMATOLACTIN RECEPTOR; SOMATOTROPIC AXIS; BINDING PROTEINS; IDENTIFICATION; CLONING; TILAPIA;
D O I
10.1007/s10695-014-0005-x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cichlasoma dimerus is a social cichlid fish capable of growing at high rates under laboratory conditions, but knowledge on somatic growth regulation is still unclear. Growth hormone (GH)/insulin-like growth factor I (IGF-I) axis is the key regulator of somatic growth in vertebrates. Two types of growth hormone receptors have been described in teleost fish, named GH receptor type 1 (GHR1) and type 2 (GHR2). In addition, isoforms of these receptors lacking part of the intracellular region have been described. The aim of this study was to evaluate the somatic growth, liver histology and changes in the GH/IGF-I axis after 4 weeks of food deprivation in C. dimerus. Four-week fasted fish showed reductions in specific growth rates in body weight (p < 0.001) and standard length (p < 0.001). Additionally, the hepatosomatic index (p < 0.001) and hepatocyte area (p < 0.001) decreased in fasted fish, while no changes in glucose levels were detected in plasma. The starvation protocol failed to induce changes in GH mRNA levels in the pituitary and IGF-I mRNA levels in liver. In contrast, IGF-I mRNA levels in muscle decreased in fasted fish (p = 0.002). On the other hand, GHR2 (detected with primer sets designed over the extracellular and intracellular region) was upregulated by starvation both in liver and muscle (p < 0.05), while GHR1 remained unchanged. These results show that a fasting period reduced somatic growth both in length and body weight concomitantly with alterations on liver and muscle GHR2 and muscle IGF-I mRNA expression.
引用
收藏
页码:51 / 60
页数:10
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