Phenolic-antioxidant capacity of mango seed kernels: therapeutic effect against viper venoms

被引:44
作者
Abdel-Aty, Azza M. [1 ]
Salama, Walaa H. [1 ]
Hamed, Mohamed Belal [1 ]
Fahmy, Afaf S. [1 ]
Mohamed, Saleh A. [1 ]
机构
[1] Natl Res Ctr, Mol Biol Dept, Cairo, Egypt
来源
REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY | 2018年 / 28卷 / 05期
关键词
Antioxidant; Anti-venom; Egyptian mango; Oxidative stress; Phenolic compounds; MANGIFERA-INDICA L; SNAKE-VENOMS; ASSAY; PRODUCTS; EXTRACT; HESPERIDIN; PEEL;
D O I
10.1016/j.bjp.2018.06.008
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
In this study, mango seed kernels extract contained a considerable amount of phenolics and flavonoids (17,400 and 3325 mg/100 g seed, respectively). The HPLC profiling revealed that hesperidin was the major phenolic compound of the mango seed kernels extract. This is the first report find hesperidin in mango extracts. The phenolic compounds of mango seed kernels extract were effective in scavenging free radicals of DPPH and ABTS with IC50 values of 47.3 and 7.9 mu g/ml, respectively. The total antioxidant activity of mango seed kernels extract based on the reduction of molybdenum was also measured. The phenolic compounds of mango seed kernels extract potentially inhibited the protease, fibrinogenase, phospholipase A(2), L-amino acid oxidase, hyaluronidase, and hemolytic activities of the most dangerous Cerastes cerastes and Echis coloratus viper venoms. The phenolic compounds of mango seed kernels extract could completely neutralize the hemorrhage and lethality of both venoms in experimental animals. It could be concluded that the mango seed kernels extract phenolic compounds with potential antioxidant activity are considered as a new avenue in the viper bite treatment. (C) 2018 Sociedade Brasileira de Farmacognosia. Published by Elsevier Editora Ltda.
引用
收藏
页码:594 / 601
页数:8
相关论文
共 47 条
[1]   Egyptian mango by-product 1. Compositional quality of mango seed kernel [J].
Abdalla, Ahmed E. M. ;
Darwish, Saeid M. ;
Ayad, Eman H. E. ;
El-Hamahmy, Reham M. .
FOOD CHEMISTRY, 2007, 103 (04) :1134-1140
[2]  
Ajila C. M., 2013, J FUNCT FOODS, V202, P11
[3]   AN INDIRECT HEMOLYTIC ASSAY FOR ASSESSING ANTIVENOMS [J].
ALABDULLA, IH ;
SIDKI, AM ;
LANDON, J .
TOXICON, 1991, 29 (08) :1043-1046
[4]   Evaluation of antioxidant and antibacterial activities of Ficus microcarpa L. fil. extract [J].
Ao, Changwei ;
Li, Anping ;
Elzaawely, Abdenaser A. ;
Xuan, Tran D. ;
Tawata, Shinkichi .
FOOD CONTROL, 2008, 19 (10) :940-948
[5]   Effects of N-acetyl-L-cysteine on redox status and markers of renal function in mice inoculated with Bothrops jararaca and Crotalus durissus terrificus venoms [J].
Barone, Juliana Marton ;
Frezzatti, Rodrigo ;
Silveira, Paulo Flavio .
TOXICON, 2014, 79 :1-10
[6]   Novel in vitro assays for assessing the haemorrhagic activity of snake venoms and for demonstration of venom metalloproteinase inhibitors [J].
Bee, A ;
Theakston, RDG ;
Harrison, RA ;
Carter, SD .
TOXICON, 2001, 39 (09) :1429-1434
[7]   Screening of phenolic compounds in by-product extracts from mangoes (Mangifera indica L.) by HPLC-ESI-QTOF-MS and multivariate analysis for use as a food ingredient [J].
Dorta, Eva ;
Gonzalez, Monica ;
Gloria Lobo, M. ;
Sanchez-Moreno, Concepcion ;
de Ancos, Begona .
FOOD RESEARCH INTERNATIONAL, 2014, 57 :51-60
[8]   Using drying treatments to stabilise mango peel and seed: Effect on antioxidant activity [J].
Dorta, Eva ;
Gloria Lobo, M. ;
Gonzalez, Monica .
LWT-FOOD SCIENCE AND TECHNOLOGY, 2012, 45 (02) :261-268
[9]   Chemistry and pharmacology of the Citrus bioflavonoid hesperidin [J].
Garg, A ;
Garg, S ;
Zaneveld, LJD ;
Singla, AK .
PHYTOTHERAPY RESEARCH, 2001, 15 (08) :655-669
[10]  
Girish KS, 2011, CURR TOP MED CHEM, V11, P2494