A pathology-MRI study of the short-T2 component in formalin-fixed multiple sclerosis brain

被引:145
作者
Moore, GRW
Leung, E
MacKay, AL
Vavasour, IM
Whittall, KP
Cover, KS
Li, DKB
Hashimoto, SA
Oger, J
Sprinkle, TJ
Paty, DW
机构
[1] Univ British Columbia, Dept Pathol & Lab Med, Vancouver, BC V5Z 1M9, Canada
[2] Univ British Columbia, Dept Radiol, Vancouver, BC V5Z 1M9, Canada
[3] Univ British Columbia, Dept Phys, Vancouver, BC, Canada
[4] Univ British Columbia, Dept Med Neurol, Vancouver, BC, Canada
[5] Vancouver Hosp & Hlth Sci Ctr, Dept Pathol & Lab Med, Vancouver, BC V5Z 1M9, Canada
[6] Vancouver Hosp & Hlth Sci Ctr, Dept Radiol, Vancouver, BC V5Z 1M9, Canada
[7] Vancouver Hosp & Hlth Sci Ctr, Dept Med Neurol, Vancouver, BC V5Z 1M9, Canada
[8] Vet Affairs Med Ctr, Res Serv, Augusta, GA USA
关键词
D O I
10.1212/WNL.55.10.1506
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
Objective: To determine the pathologic basis of areas not exhibiting signal of the short-T2 component of the T2 relaxation distribution in MS, as studied in formalin-fixed brain. Background: A myelin-specific MRI signal would be of great importance in assessing demyelination in patients with MS. Evidence indicates that the short-T2 (10 to 50 millisecond) component of the T2 relaxation distribution originates from water in myelin sheaths. The authors present two cases of MS in which the anatomic distribution of the short-T2 component was correlated with the pathologic findings in postmortem formalin-fixed brain. Method: One half of the formalin-fixed brain was suspended in a gelatin-albumin mixture cross-linked with glutaraldehyde, and scanned with a 32-echo MRI sequence. The brain was then cut along the center of the 5-mm slices scanned, photographed, dehydrated, and embedded in paraffin. Paraffin sections, stained with Luxol fast blue and immunocytochemically for 2',3'-cyclic nucleotide 3'-phosphohydrolase for myelin and by the Bielschowsky technique for axone, were compared with the distribution of the amplitude of the short-T2 component of the comparable image slices. Results: The anatomic distribution of the short-T2 component signal corresponded to the myelin distribution. Chronic, silent MS plaques with myelin loss correlated with areas of absence of short-T2 signal. The numbers of axone within lesions were reduced, but many surviving axons were also seen in these areas of complete loss of myelin. Conclusion: In formalin-fixed MS brains the short-Ta component of the T2 relaxation distribution corresponds to the anatomic distribution of myelin. Chronic, silent demyelinated MS plaques show absence of the short-Ta component signal. These results support the hypothesis that the short-T2 component originates from water related to myelin.
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页码:1506 / 1510
页数:5
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