Biochemical and genetic analysis of the yeast proteome with a movable ORF collection

被引：364
|
作者：
Gelperin, DM
White, MA
Wilkinson, ML
Kon, Y
Kung, LA
Wise, KJ
Lopez-Hoyo, N
Jiang, LX
Piccirillo, S
Yu, HY
Gerstein, M
Dumont, ME
Phizicky, EM
Snyder, M [1 ]
Grayhack, EJ
机构：
[1] Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT 06520 USA
[2] Yale Univ, Dept Biochem & Biophys, New Haven, CT 06520 USA
[3] Univ Rochester, Sch Med & Dent, Dept Biochem & Biophys, Rochester, NY 14642 USA
来源：
GENES & DEVELOPMENT | 2005年 / 19卷 / 23期
关键词：
biochemical genomics; protein microarray; proteome; high-throughput expression; galactose lethality; glycosylation;
D O I：
10.1101/gad.1362105
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Functional analysis of the proteome is an essential part of genomic research. To facilitate different proteomic approaches, a MORF (moveable ORF) library of 5854 yeast expression plasmids was constructed, each expressing a sequence-verified ORF as a C-terminal ORF fusion protein, under regulated control. Analysis of 5573 MORFs demonstrates that nearly all verified ORFs are expressed, suggests the authenticity of 48 ORFs characterized as dubious, and implicates specific processes including cytoskeletal organization and transcriptional control in growth inhibition caused by overexpression. Global analysis of glycosylated proteins identifies 109 new confirmed N-linked and 345 candidate glycoproteins, nearly doubling the known yeast glycome.
引用
收藏
页码：2816 / 2826
页数：11
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