Effects of Various Adhesive Substrates on the Adhesion Forces of Endothelial Progenitor Cells

被引：5

作者：

Wang, Guixue ^{[1
]}

Xiao, Li ^{[1
]}

Wu, Xue ^{[1
]}

Xie, Xiang ^{[1
]}

Tang, Chaojun ^{[1
]}

Yang, Li ^{[1
]}

Lee, James C-M. ^{[2
]}

机构：

[1] Chongqing Univ, Key Lab Biorheol Sci & Technol, Minst Educ, Chongqing Engn Lab Vasc Implants,Bioengn Coll, Chongqing 400044, Peoples R China

[2] Univ Missouri, Dept Biol Engn, Columbia, MO 65211 USA

来源：

JOURNAL OF MEDICAL AND BIOLOGICAL ENGINEERING | 2012年 / 32卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Endothelial progenitor cell; Adhesive substrate; Single micropipette aspiration technique; Adhesion forces; CD133; antibody; ELUTING STENT; FIBRONECTIN; RESTENOSIS; NANOMETER; SURFACES; ANTIBODY; CAPTURE; TARGET;

D O I：

10.5405/jmbe.855

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

This study evaluates the effects of various substrates on the adhesion forces of endothelial progenitor cells (EPCs) and identifies the optimal antibody for EPC-capture stents. Single-cell micropipette aspiration (SCMA) is employed to measure the adhesion forces of single EPCs on various substrates, namely gelatin and antibodies CD34, VEGFR-2, and CD133. The adhesion forces of single EPCs on each substrate are compared with those of human umbilical vein endothelial cells (HUVECs). Compared to HUVECs, our results show that EPCs exhibit better adhesion on gelatin, anti-CD34, and anti-CD133. The adhesion forces of EPCs or HUVECs on surfaces coated with these three substances increases with the concentration of these three substances. The adhesion forces are cell-type-and substrate-dependent. EPCs have stronger adhesion than HUVECs on surfaces coated with anti-CD34 or anti-CD133. The adhesion forces of EPCs on an anti-CD133-coated surface are higher than those on anti-CD34-, anti-VEGFR-2-, and gelatin-coated surfaces. The findings suggest that using CD133 antibody to capture EPCs is more efficient than using CD34 antibody.

引用

页码：70 / 75

页数：6

共 29 条

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