Potential threat of a new pathotype of Papaya leaf distortion mosaic virus infecting transgenic papaya resistant to Papaya ringspot virus

被引：26

作者：

Bau, H. J. ^{[2
]}

Kung, Y. -J. ^{[1
]}

Raja, J. A. J. ^{[1
]}

Chan, S. -J. ^{[1
]}

Chen, K. -C. ^{[1
]}

Chen, Y. -K. ^{[1
]}

Wu, H. -W. ^{[1
]}

Yeh, S. -D. ^{[1
]}

机构：

[1] Natl Chung Hsing Univ, Dept Plant Pathol, Taichung 40227, Taiwan

[2] Transworld Inst Technol, Dept Biotechnol, Yunlin, Taiwan

来源：

PHYTOPATHOLOGY | 2008年 / 98卷 / 07期

关键词：

D O I：

10.1094/PHYTO-98-7-0848

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A virus identified as a new pathotype of Papaya leaf distortion mosaic virus (PLDMV, P-TW-WF) was isolated from diseased papaya in an isolated test-field in central Taiwan, where transgenic papaya lines resistant to Papaya ringspot virus (PRSV) were evaluated. The infected plants displayed severe mosaic, distortion and shoe-stringing on leaves; stunting in apex; and water-soaking on petioles and stems. This virus, which did not react in enzyme-linked immunosorbent assay with the antiserum to the PRSV coat protein, infected only papaya, but not the other IS plant species tested. Virions studied under electron microscope exhibited mor-phology and dimensions of potyvirus particles. Reverse transcription-polymerase chain reaction conducted using potyvirus-specific primers generated a 1,927-nucleotide product corresponding to the 3' region of a potyvirus, showing high sequence identity to the CP gene and 3' non-coding region of PLDMV. Search for similar isolates with the antiserum against CP of P-TW-WF revealed scattered occurrence of PLDMV in Taiwan. Phylogenetic analysis of PLDMV isolates of Taiwan and Japan indicated that the Taiwan isolates belong to a separate genetic cluster. Since all the Taiwan isolates infected only papaya, unlike the cucurbit-infecting Japanese P type isolates, the Taiwan isolates are considered a new pathotype of PLDMV. Susceptibility of all our PRSV-resistant transgenic papaya lines to PLDMV indicates that the virus is an emerging threat for the application of PRSV-resistant transgenic papaya in Taiwan and elsewhere.

引用

页码：848 / 856

页数：9

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