Transmigration of human neutrophils across airway epithelial cell monolayers is preferentially in the physiologic basolateral-to-apical direction

被引:39
|
作者
Liu, L
Mul, FPJ
Lutter, R
Roos, D
Knol, EF
机构
[1] NETHERLANDS RED CROSS, BLOOD TRANSFUS SERV, CENT LAB, NL-1066 CX AMSTERDAM, NETHERLANDS
[2] UNIV AMSTERDAM, EXPT & CLIN IMMUNOL LAB, AMSTERDAM, NETHERLANDS
[3] UNIV AMSTERDAM, ACAD MED CTR, DEPT PULMONOL, NL-1105 AZ AMSTERDAM, NETHERLANDS
关键词
D O I
10.1165/ajrcmb.15.6.8969272
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
To study the mechanisms involved in the movement of neutrophils from the blood stream into the lung airways, we investigated human neutrophil transmigration across a monolayer of human airway epithelial cells, both in the apical-to-basolateral direction and in the more physiologic basolateral-to-apical direction. Migration of human neutrophils across monolayers of human airway epithelial H292 cell-line cells and primary bronchial epithelial cells occured most efficiently in the basolateral-to-apical direction, both after the addition of chemoattractants to resting epithelial cells and across interleukin-1 beta (IL-1 beta)-stimulated epithelial cells. Blocking studies with monoclonal antibodies revealed that the migration of neutrophils was mediated by the CR3 adhesion molecule (CD11b/CD18) on the neutrophils. IL-1 beta-treated epithelial cells caused neutrophil movement via the secretion of chemoattractants. The most potent chemoattractant released by the epithelial cells was found to be IL-8, because the IL-1 beta-induced migration was inhibited for 75 +/- 10% by the addition of an antibody against IL-8. After apical stimulation of the epithelial cells with an optimal concentration ofIL-1 beta, 27 +/- 4 ng/ml IL-8 was found in the supernatant at the apical side of epithelial cells. Platelet-activating factor (PAF) synthesis by the epithelial cells did not play a role in neutrophil transmigration, as was demonstrated by the lack of inhibition of this process after addition of the PAF-receptor antagonist WEB 2086. We conclude that the movement of neutrophils across airway epithelial cell monolayers occurs preferentially in the physiologic basolateral-to-apical direction, indicating that the polarity of epithelial cells is important for neutrophil transmigration.
引用
收藏
页码:771 / 780
页数:10
相关论文
共 50 条
  • [1] MIGRATION OF HUMAN NEUTROPHILS ACROSS BRONCHIAL EPITHELIUM IN BASOLATERAL TO APICAL DIRECTION
    SHARMA, GD
    INFELD, MD
    DAVIS, PB
    PEDIATRIC RESEARCH, 1994, 35 (04) : A397 - A397
  • [2] LIPOXIN A(4) MODULATES TRANSMIGRATION OF HUMAN NEUTROPHILS ACROSS INTESTINAL EPITHELIAL MONOLAYERS
    COLGAN, SP
    SERHAN, CN
    PARKOS, CA
    DELPARCHER, C
    MADARA, JL
    JOURNAL OF CLINICAL INVESTIGATION, 1993, 92 (01): : 75 - 82
  • [3] Neutrophil transmigration across human airway epithelial monolayers - Mechanisms and dependence on electrical resistance
    Kidney, JC
    Proud, D
    AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 2000, 23 (03) : 389 - 395
  • [4] Characterization of basolateral-to-apical transepithelial transport of cadmium in intestinal TC7 cell monolayers
    Pascale Carrière
    Marc Mantha
    Sophie Champagne-Paradis
    Catherine Jumarie
    BioMetals, 2011, 24 : 857 - 874
  • [5] Characterization of basolateral-to-apical transepithelial transport of cadmium in intestinal TC7 cell monolayers
    Carriere, Pascale
    Mantha, Marc
    Champagne-Paradis, Sophie
    Jumarie, Catherine
    BIOMETALS, 2011, 24 (05) : 857 - 874
  • [6] Aspergillus fumigatus Cell Wall Promotes Apical Airway Epithelial Recruitment of Human Neutrophils
    Feldman, Michael B.
    Dutko, Richard A.
    Wood, Michael A.
    Ward, Rebecca A.
    Leung, Hui Min
    Snow, Ryan F.
    De La Flor, Denis J.
    Yonker, Lael M.
    Reedy, Jennifer L.
    Tearney, Guillermo J.
    Mou, Hongmei
    Hurley, Bryan P.
    Vyas, Jatin M.
    INFECTION AND IMMUNITY, 2020, 88 (02)
  • [7] Expression and distribution of MAL2, an essential element of the machinery for basolateral-to-apical transcytosis, in human thyroid epithelial cells
    Marazuela, M
    Martín-Belmonte, F
    García-López, MA
    Aranda, JF
    de Marco, MC
    Alonso, MA
    ENDOCRINOLOGY, 2004, 145 (02) : 1011 - 1016
  • [8] Transduction of basolateral-to-apical signals across epithelial cells: Ligand-stimulated transcytosis of the polymeric immunoglobulin receptor requires two signals
    Luton, F
    Mostov, KE
    MOLECULAR BIOLOGY OF THE CELL, 1999, 10 (05) : 1409 - 1427
  • [9] Activity and inhibition of prostasin and matriptase on apical and basolateral surfaces of human airway epithelial cells
    Nimishakavi, Shilpa
    Besprozvannaya, Marina
    Raymond, Wilfred W.
    Craik, Charles S.
    Gruenert, Dieter C.
    Caughey, George H.
    AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, 2012, 303 (02) : L97 - L106
  • [10] Glucose homeostasis across human airway epithelial cell monolayers: role of diffusion, transport and metabolism
    Kalsi, Kameljit K.
    Baker, Emma H.
    Fraser, Owen
    Chung, Yuen-Li
    Mace, Oliver J.
    Tarelli, Edward
    Philips, Barbara J.
    Baines, Deborah L.
    PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 2009, 457 (05): : 1061 - 1070