Modulation of the immune response using Rapana thomasiana hemocyanin

被引：32
作者：
Tchorbanov, Andrey [1 ]
Idakieva, Krassimira [2 ]
Mihaylova, Nikolina [1 ]
Doumanova, Lyuba [1 ]
机构：
[1] Bulgarian Acad Sci, Inst Microbiol, BU-1113 Sofia, Bulgaria
[2] Bulgarian Acad Sci, Inst Organ Chem, BU-1113 Sofia, Bulgaria
来源：
INTERNATIONAL IMMUNOPHARMACOLOGY | 2008年 / 8卷 / 07期
关键词：
adjuvant; protein-carrier; hemocyanin; Rapana thomasiana; gastropod;
D O I：
10.1016/j.intimp.2008.03.008
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
We have investigated the non-specific immunostimulatory and specific immunomodulatory effects of hemocyanin from marine gastropod Rapana thomasiana (RtH). The purified RtH, its structural subunits RtH1 and RtH2 and a construct with influenza virus hemagglutinin intersubunit peptide (IP) were used in immunization protocols of Balb/c mice. Antibody formation against RtH, RtH1, RtH2, RtH-IP as well as anti-RtH IgG antibody isotypes were determined by ELISA. The immune homology between both subunits and the whole RtH molecule was investigated by cross-blotting technique. The retaining of the B-cell epitope of IP, coupled to the RtH was recognised by Western blot. The results obtained demonstrate that the immunization with RtH or its subunits in experimental models resulted in strong immune response in vivo. Common epitope of influenza A virus hemagglutinin jointed to RtH results in generation of molecule with increased immunogenicity. Our results are the first demonstration that RtH and/or its subunits could be used in different immunization protocols as an adjuvant or as a protein-carrier. (C) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码：1033 / 1038
页数：6
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